Estudo da pluripotencialidade do fígado fetal canino nos diferentes períodos gestacionais

Abstract

The study of the differentiation capacity of some cells, called stem cells or '' sistem '' cells represents an important tool for understanding and developing new research, as well as an enormous potential for discovering the treatment of numerous diseases. These embryonic stem cells are pluripotent, capable of multiplication and differentiation, and can proliferate in several cell types depending on the culture conditions. The identification of factors that lead to the direction of the cell differentiation process will allow, from embryonic stem cells, the culture to be carried out in a controlled manner among the most different cell types, making bioengineering possible. In this work, cells of the canine fetal liver were studied. The liver performs an important hematopoietic function during fetal development, while in the adult it has a multiplicity of functions such as: bile excretion, storage, synthesis and biotransformation. The livers, for study, were obtained from fetuses with gestational age with an average of 25, 35, 45 and 55 days, from females of mixed breed dogs that underwent ovariosalpingohesterectomy. These canine fetal liver cells were analyzed histologically to characterize cell differentiation, using previously established histological methods.