Estabelecimento das condições de isolamento e cultivo das células tronco mesenquimais da médula óssea em fetos de cães

Abstract

Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into multiple differentiated terminal strains, including bone, cartilage, muscle, nerve, adipose and marrow stromal cells in vivo and in vitro. These cells are in addition to being isolated from the adult bone marrow and can be isolated from the fetal bone marrow. However, there is little study regarding the isolation technique of MSCs from fetal bone marrow. In this way, the work proposed to isolate MSC from canine fetuses based on the established methodology for obtaining MSC from adult human bone marrow. For this purpose, 12 pregnant female uteri were used to collect the medullary aspirate from the femur bone. The culture medium of choice was alpha-MEM and 15% fetal bovine serum, which provided the isolation of positive CTM-CD44.