Medicina Veterinária
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Trabalho acadêmico ou acadêmico é o texto (em sentido lato ou estrito) resultado de algum dos diversos processos ligados à produção e transmissão de conhecimento executados no âmbito das instituições ensino, pesquisa e extensão universitária, formalmente reconhecidas para o exercício dessas atividades.
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A UNIFEOB mantém uma política de pesquisa que tem como objetivo orientar as ações desenvolvidas nesse campo do conhecimento. As pesquisas têm alicerce em princípios e critérios de produção de conhecimento científico e tecnológico, seguindo normas éticas de pesquisa em seres humanos e animais e dissociada do ensino e da extensão. As atividades convergem com as diretrizes nacionais, visando o desenvolvimento sustentável social, econômico e ambiental.
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Item Membranas embrionárias e fetais de equinos sob análise de microscopia eletrônica de transmissão(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) NASCIMENTO, Priscila Leal do; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luis Rezende; FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Ana Carolina Furlanetto; CARVALHO, Ana Flávia deTo develop this research, 16 pregnant uteri were used, of which 16 placentas were obtained divided into groups of different stages of development. The material was collected from adult, mixed breed mares up to 100 days of gestation. The membranes were intended for ultra-structural analysis and the results obtained under transmission electron microscopy (MET) were similar to those found by ASSIS-NETO (2005), in bovine membranes, and ITURRIZAGA (2005), in llamas. The fetal membranes did not show relevant structural differences in the different periods studied. The chorion showed columnar epithelium; mesenchymal tissue rich in collagen fibers, the trophoblastic cells being uni, binucleated and rarely trinucleated. A large number of mitochondria were present mainly at the apex of cells. The allantoic presented a simple columnar epithelium with globular nuclei and a large concentration of mitochondria at the cell apex, in the cell base there was a large amount of rough endoplasmic reticulum. The amnion showed a simple, squamous epithelium and at the cellular apex there was a large amount of electrondense granules covered by a rounded halo. The yolk sac epithelium varied from globose to columnar, with uni or binucleate cells in a single layer, with a large amount of rough endoplasmic reticulum, uniformly distributed. Between the endodermal cells, intercellular spaces were found. So far, it is concluded that the mare's placenta is choroidal alloid, epithelial, diffuse, non-deciduous and villous, with corium cells and a yolk sac with characteristics of protein secretion, while amnion presented secretion vesicles, but with less amount of rough endoplasmic reticulum.Item Estabelecimento do protocolo Agnor para membranas embrionárias e fetais de equinos(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) MANÇANARES, Ana Carolina Furlanetto; MANÇANARES, Celina Almeida Furlanetto; FERRAZ, Ana Cláudia Cristiane; CARVALHO, Ana Flávia deThe nucleolus organizing regions (RONs) or Nucleolar Organizer Regions (NORs) are slightly stained chromatin regions around which, at the end of the telophase, the nucleolus is formed again after mitosis. A peculiar group of acidic proteins that have a high affinity for silver, are located in the same places as NORs, which gives the same characteristics of being clearly and quickly visualized by stains using silver nitrate. For this study, equine embryo / fetus membranes obtained from 30 placentas were used. Such material was collected from adult, mixed breed mares and at different gestation periods of up to 100 days. Fragments of the embryo / fetus membranes were fixed in 10% formaldehyde for routine histological procedures (TOLOSA et al, 2003). Serial cuts with a thickness of 5μm were obtained in a microtome (Leica 2165®,) which were dewaxed and immersed in a final solution 1: 2, consisting of colloidal solution (gelatin 2% and formic acid 1%) and silver nitrate solution (AgNO3) (1: 1). They were taken to the oven at 37 ° C, in a humid chamber, for approximately 20 minutes. After washing the slides with water, a 1% sodium thiosulfate solution was applied for one minute and followed by washing and drying at room temperature, being stored in a darkroom. After the silver impregnation staining of the NORs (Nucleolar Organizer Regions) some slides were subjected to Eosin baths so that the cytoplasm was stained and thus obtaining easier cell individualization. The results obtained were appropriate, showing the appropriate protocol, and the eosin background provided a better visualization of cell delimitations, thus facilitating the possible counting of NORs per cell.Item Análise macroscópica dos anexos embrionários e fetais de equinos em até 107 dias de gestação(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia deThe horse (Equus caballus, Linneaus 1758) is a large ungulate mammal of the Equidea family, one of the seven modern species of the Equus genus. Domesticated for thousands of years, it is an unparalleled animal, of unusual beauty, grace, sensitivity and athletic ability; a fascinating animal for study, as described by Evans et al. (nineteen ninety); Ginther (1992) and Dantzer et al. (1998). Because it is not a simple model, it offers a comprehensive range for research and challenges scientists. Equine placentation and its embryonic development are some of the issues to be explored. Thus, this study aimed to describe microscopically the embryonic attachments of the embryo / fetus of horses in the first four months of pregnancy. In order to carry out this research, 37 placentas of adult, mixed breed mares were collected and divided into groups at the different stages of development, following the methodology employed by EVANS SACK (1973), until 107 days of gestation, and were subsequently destined for the microscopy of light. The membrane fragments (corium, allantois, amnion and yolk sac) were fixed in 10% formalin and Bouin followed by routine paraffin processing (TOLOSA et al; 2003). Serial slices with a thickness of 5μm were obtained in a microtome (Leica 2165®) which were submitted to staining by the methods of hematoxylin and eosin, picrosirius, Toluidine blue and Masson's trichrome (TOLOSA et al; 2003). In the four groups, the amnion presented with a simple pavement epithelium and mesenchyme without any vascularization, only vascularization was evident when the amnion fused with the allantois, which is also a simple pavement epithelium. The corium presented villi with a low columnar epithelium and brushed edges, uni and binucleated cells, highly vascularized, and the size and quantity of villi differed from the groups. The yolk sac presented an epithelium whose cells were large and globular, resting on the mesenchyme, forming vascular islands full of hemangioblasts, being non-existent in animals from 47 to 107 days of gestation.Item Análise macroscópica do aparelho reprodutor masculino do ouriço cacheiro(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) CASALS, Juliana Barbosa; BARREIRO, Juliana Regina; ROSA, Ricardo Alexandre; MANÇANARES, Celina Almeida Furlanetto; FAVARON, Phelipe Oliveira; CARVALHO, Ana Flávia de; MIGLINO, Maria Angélica; MARQUES, Leandro de Oliveira; ANDRADE, Filipe Ian Bindez de; NASCIMENTO, Priscila Leal do; FERRAZ, Ana Cláudia Cristiane; AMBRÓSIO, Carlos EduardoRodents comprise most of the mammalian class. There are currently 2,021 species, organized into 443 genera and 29 families. Hedgehogs (Coendou villosus), are animals that in captivity, reproduce with some ease and become tame. In the urban and metropolitan region their presence is becoming common and this can be identified by the number of accidents involving dogs in the yard, registered in veterinary clinics. This work aimed to describe macroscopically the reproductive apparatus of Coendou villosus (hedgehog) to better clarify the anatomy of this important organ in the wild rodent model, since very little is known about this species. We compared the results obtained with other rodent species already studied. For the macroscopic description of the male reproductive system, 2 animals from donations were used, these animals ended up dying after attack by dogs.Item Estudo macroscópico de estômago de Mocó(Centro Universitário da Fundação de Ensino Octávio Bastos, 2008-11-30) FERRAZ, Ana Cláudia Cristiane; ROSA, Felipe Gonçalves; MANÇANARES, Celina de Almeida Furlanetto; SILVA, Angélica do Rocio Carvalho; AMBRÓSIO, Carlos Eduardo; OLIVEIRA, Moacir Franco; ROQUETO, Marco Antônio; ROSA, Ricardo Alexandre; CARVALHO, Ana Flávia deFour stomachs were used for macroscopic analysis. Stomachs were measured, photographed and dissected according to biometry and anatomy techniques. The 4 stomachs were similar in terms of their external and internal anatomical characteristics, with greater, lesser curvature and cardiac, funicular and pyloric regions, respectively. The shape was also similar to that of other domestic animals, and there was no statistically significant difference between the equal regions measured or in terms of their height, however, there was variation between two different structures (greater and lesser curvature). It was concluded that the stomachs of mocós are anatomically similar to those of other domestic monogastrics and rodents already studied.Item Análise biométrica de embriões, fetos e das membranas placentárias de equinos.(Centro Universitário da Fundação de Ensino Octávio Bastos, 2008-11-30) FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia deFor this study, embryos, fetuses and attached membranes of 23 concepts of mares from Frigorífico Miramar - Pelotas RS were used. Embryos and fetuses were analyzed for size and external developmental characteristics to analyze gestational age. The attached membranes were weighed and measured for later correlation with gestational ages. The results obtained so far indicate that the embryonic and fetal development of horses is very fast in the early stages of development, this being a critical period of placental implantation and the beginning of the endocrine activities of the placenta. The biometric data related to the umbilical cord are not progressive according to development, having shown great variations among the animals studied.