12º Encontro Acadêmico de Produção Científica
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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Item Estabelecimento das condições de isolamento e cultivo das células tronco mesenquimais da médula óssea em fetos de cães(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) BORGHESI, Jéssica; MARIO, Lara Carolina; MELLO, Ana Carolina Landentin; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane ValverdeMesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into multiple differentiated terminal strains, including bone, cartilage, muscle, nerve, adipose and marrow stromal cells in vivo and in vitro. These cells are in addition to being isolated from the adult bone marrow and can be isolated from the fetal bone marrow. However, there is little study regarding the isolation technique of MSCs from fetal bone marrow. In this way, the work proposed to isolate MSC from canine fetuses based on the established methodology for obtaining MSC from adult human bone marrow. For this purpose, 12 pregnant female uteri were used to collect the medullary aspirate from the femur bone. The culture medium of choice was alpha-MEM and 15% fetal bovine serum, which provided the isolation of positive CTM-CD44.Item Análise morfológica das células do aspirado medular de fetos caninos(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) MÁRIO, Lara Carolina; BORGHESI, Jéssica; MELLO, Ana Carolina Landentin; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane ValverdeThe adult bone marrow is a source of stem cells (CT), which include the hematopoietic stem cells (HSC) that originate the entire definitive hematopoietic lineage; and mesenchymal stem cells (MSC), and or stromal which are responsible for providing a favorable environment for hematopoiesis. In addition to these main cell types, bone marrow is composed of several parents, differentiated cells and an extracellular matrix. This environment where CT is found is called a niche, as it houses a large amount of CT and cellular progenitors that are kept quiescent, acting normally and or in the face of injuries. However, little is known about the fetal bone marrow niche. It is known that the bone marrow niche is derived during the ontogenesis of the yolk sac, which subsequently migrates to various hematopoietic organs such as the placenta and liver, ultimately settling in the bone marrow. Thus, the present work aims to describe morphologically the cell types of canine fetal bone marrow at 40 and 60 days of gestation. For this purpose, 6 pregnant female uteri were used to collect the medullary aspirate from the femur bone and later structural and ultrastructural morphological analysis. The results demonstrated that the canine fetal bone marrow at 60 days of gestation has cell types similar to that of the adult bone marrow as well as CT, thus being able to be used as a source of CT isolation.Item Descrição microscópica do sistema reprodutor feminino do Tamanduá Mirim(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) OLIO, Renan Lopes; LOBO, Luis Miguel; WENCESLAU, Cristiane Valverde; MANÇANARES, Celina Almeida Furlanetto; BRANCO, Érika Renata; GARCIA, Rafael de Carvalho; LIMA, Ana Rita de; CARVALHO, Ana Flávia deThe juvenile anteater is an extremely captivating and docile animal, belongs to the Xenarthra Order, Family Myrmecophagidae, which are toothless members, and therefore called edentats. As it is an animal of our fauna, data about the species, with regard to morphology and physiological aspects, are very scarce, and generally focus on diseases and external parasites, from capture and expeditions. For this study, two animals donated by UFRA – PA were used, in which the macroscopic characteristics of the female reproductive system were analyzed, in order to clarify the morphological aspects of the female reproductive system, peculiar to the species, in order to assist in their preservation and create reproductive biotechnology tools. The obtained results reveal that the ovaries are longilinear, the uterine tubes tortuous, and the simple uterus in pear shape, as well as in the sloth, in the woman and in the primates. The cervix is quite thick and the vagina communicates with the vestibule and the vulva.Item Anatomia microscópica do sistema reprodutor feminino do Tamanduá Bandeira(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) GARCIA, Rafael de Carvalho; BRANCO, Èrika Renata; LIMA, Ana Rita de; OLIO, Rennan Lopes; WENCESLAU, Cristiane Valverde; MANÇANARES, Celina Almeida Furlanetto; THOMAZ, Juliana Mantovani; CARVALHO, Ana Flávia deThe giant anteater is a large animal and can be very aggressive with its claws if disturbed, it belongs to the Xenarthra Order, Family Myrmecophagidae, which are toothless members, and therefore called edentats. Some reports have been made about this species, but many relevant data have not been published, emphasizing basic concepts of morphology and biometrics, which could be extremely useful for the development of reproductive biotechnologies, aiming at the preservation of the species. For this study, two animals donated by UFRA – PA were used, in which the microscopic characteristics of the female reproductive system were analyzed, in order to clarify the peculiar aspects of the species. The results obtained reveal that the ovaries, fallopian tubes and uterus are similar histologically to those of domestic animals.