10º Encontro Acadêmico de Produção Científica
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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Item Desenvolvimento embrionário do fígado e do pâncreas de embriões e fetos bovinos(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) SANTOS, Ana Paula de Almeida; MANÇANARES, Celina Almeida Furlanetto; CARVALHO, Ana Flávia de; ROSA, Ricardo AlexandreIn domestic mammals, the increase in production rates as well as the greater dissemination of superior genetic material in herds, has been the subject of intense studies and investments, which has allowed a great advance and development of several biotechnologies related to animal reproduction. In cattle, most organs and body parts are formed between the 2nd and 6th week of gestation. During this period, the digestive tract, lungs, liver and pancreas develop from the primitive intestine, and the beginnings of the muscular, skeletal, nervous and urogenital system are established. In the course of development, the epithelial components of the digestive tract are derived from the endoderm that lines the primitive intestine and the connective and muscular components. Approximately 25 to 45% of bovine embryos are lost until the final implantation process. Due to the scarce literature related to organogenesis and the growth of technological innovations linked to bovine reproduction, the present study aims to study based on the normal development of the pancreas and liver of bovine embryos, thus generating a comparison for the understanding of numerous deformities and anomalies frequently encountered.Item Estudo macroscópico do nervo isquiático do Quati (Nasua nasua) e suas ramificações(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) SANTOS, Amilton César dos; MANÇANARES, Ana Carolina Furlanetto; BERTOSSOLI, Bruno Machado; CARLOS, Camila Zeferino; SILVA, Flávia Martins da; BENATO, Talita Mascarim; OLIVEIRA, Vanessa Cristina de; ROSA, Ricardo Alexandre; CARVALHO, Ana Flávia de; MANÇANARES, Celina Almeida FurlanettoThe coati (Nasua nasua) is a procionid with a flexible snout, small eyes and ears, which have long canines. They are omnivorous animals with diurnal, terrestrial and arboreal habits, which feed mainly on fruits and small invertebrates. This omnivore has been constituted in an important group among the animals submitted to different studies, which is extremely important even for the understanding of its own evolution, adding to the fact that detailed knowledge of its Anatomy can represent an important factor for their preservation and protection. The objective of this research is, is to know the anatomical aspects of the sciatic nerve, its origin and composition, aiming at a better understanding of the innervation of the pelvic limb of this animal. For this work, 3 orthothanged animals were used, from the Scientific Creatory (CECRIMPAS) -UNIFEOB authorized by IBAMA as the Process: nº 02027.003731 / 04-76. The techniques used were latex injection into the abdominal aorta to replenish the arterial bed, followed by fixation in a 10% formaldehyde solution. The sciatic nerve and its branches were dissected, measured and photo documented. After analyzing the pieces studied, we could observe that the largest nerve in the coati's body is the sciatic nerve, as well as in the feline, swine and goat. This one The nerve derives its fibers essentially from the ventral branches of the lumbar nerves and from the sacral nerves that make up the lumbosacral plexus.Item Morfologia dos músculos superficiais do Quati (Nasua nasua)(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) SANTOS, Amilton César dos; OLIVEIRA, Vanessa C. de; ROSA, Ricardo Alexandre; CARVALHO, Ana Flávia de; MANÇANARES, Celina Almeida FurlanettoThe coati is an animal that belongs to the Phylum Chordata, the Mammalia Class, the Carnivorous Order and the Procyonidae Family. The striking feature of the Procyonidae family is the presence of five digits in the paws, that is, these animals are classified as plantigrade, being able to perform manual movements in different directions. He has habits of climbing trees to breed, escape danger and stay overnight. It feeds on fruits, small vertebrates, insects, nectar, eggs and vegetables. For this work, 3 orthothanized animals were used, coming from the Scientific Creatory (CECRIMPAS) - UNIFEOB authorized by IBAMA as Process: nº 02027.003731 / 04-76. The techniques used were latex injection into the abdominal aorta to replenish the arterial bed, followed by fixation in a 10% formaldehyde solution. Muscles are individualized structures that cross one or more joints and by their contraction are able to transmit movements. In the coati the superficial muscles are: platysma, cutaneous trunk, orbicularis eye, orbicularis mouth, zygomatic, masseter, nasolabial lifter, pectoralis, abdominal, deltoid, brachycephalic, great dorsal, triceps brachial, biceps brachii, extensors, flexors, brachial, semitendinosus, semimembranosus, sartorius, biceps femoris, quadriceps, tensor fascia lata, gluteus. In this species studied, anatomical adaptations were found in the brachial and pelvic muscles that developed more than the other species (Puma, Alpaca, Dog, Cat), due to their climbing habit.Item Membranas embrionárias e fetais de equinos sob análise de microscopia eletrônica de transmissão(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) NASCIMENTO, Priscila Leal do; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luis Rezende; FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Ana Carolina Furlanetto; CARVALHO, Ana Flávia deTo develop this research, 16 pregnant uteri were used, of which 16 placentas were obtained divided into groups of different stages of development. The material was collected from adult, mixed breed mares up to 100 days of gestation. The membranes were intended for ultra-structural analysis and the results obtained under transmission electron microscopy (MET) were similar to those found by ASSIS-NETO (2005), in bovine membranes, and ITURRIZAGA (2005), in llamas. The fetal membranes did not show relevant structural differences in the different periods studied. The chorion showed columnar epithelium; mesenchymal tissue rich in collagen fibers, the trophoblastic cells being uni, binucleated and rarely trinucleated. A large number of mitochondria were present mainly at the apex of cells. The allantoic presented a simple columnar epithelium with globular nuclei and a large concentration of mitochondria at the cell apex, in the cell base there was a large amount of rough endoplasmic reticulum. The amnion showed a simple, squamous epithelium and at the cellular apex there was a large amount of electrondense granules covered by a rounded halo. The yolk sac epithelium varied from globose to columnar, with uni or binucleate cells in a single layer, with a large amount of rough endoplasmic reticulum, uniformly distributed. Between the endodermal cells, intercellular spaces were found. So far, it is concluded that the mare's placenta is choroidal alloid, epithelial, diffuse, non-deciduous and villous, with corium cells and a yolk sac with characteristics of protein secretion, while amnion presented secretion vesicles, but with less amount of rough endoplasmic reticulum.Item Estabelecimento do protocolo Agnor para membranas embrionárias e fetais de equinos(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) MANÇANARES, Ana Carolina Furlanetto; MANÇANARES, Celina Almeida Furlanetto; FERRAZ, Ana Cláudia Cristiane; CARVALHO, Ana Flávia deThe nucleolus organizing regions (RONs) or Nucleolar Organizer Regions (NORs) are slightly stained chromatin regions around which, at the end of the telophase, the nucleolus is formed again after mitosis. A peculiar group of acidic proteins that have a high affinity for silver, are located in the same places as NORs, which gives the same characteristics of being clearly and quickly visualized by stains using silver nitrate. For this study, equine embryo / fetus membranes obtained from 30 placentas were used. Such material was collected from adult, mixed breed mares and at different gestation periods of up to 100 days. Fragments of the embryo / fetus membranes were fixed in 10% formaldehyde for routine histological procedures (TOLOSA et al, 2003). Serial cuts with a thickness of 5μm were obtained in a microtome (Leica 2165®,) which were dewaxed and immersed in a final solution 1: 2, consisting of colloidal solution (gelatin 2% and formic acid 1%) and silver nitrate solution (AgNO3) (1: 1). They were taken to the oven at 37 ° C, in a humid chamber, for approximately 20 minutes. After washing the slides with water, a 1% sodium thiosulfate solution was applied for one minute and followed by washing and drying at room temperature, being stored in a darkroom. After the silver impregnation staining of the NORs (Nucleolar Organizer Regions) some slides were subjected to Eosin baths so that the cytoplasm was stained and thus obtaining easier cell individualization. The results obtained were appropriate, showing the appropriate protocol, and the eosin background provided a better visualization of cell delimitations, thus facilitating the possible counting of NORs per cell.Item Análise macroscópica dos anexos embrionários e fetais de equinos em até 107 dias de gestação(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia deThe horse (Equus caballus, Linneaus 1758) is a large ungulate mammal of the Equidea family, one of the seven modern species of the Equus genus. Domesticated for thousands of years, it is an unparalleled animal, of unusual beauty, grace, sensitivity and athletic ability; a fascinating animal for study, as described by Evans et al. (nineteen ninety); Ginther (1992) and Dantzer et al. (1998). Because it is not a simple model, it offers a comprehensive range for research and challenges scientists. Equine placentation and its embryonic development are some of the issues to be explored. Thus, this study aimed to describe microscopically the embryonic attachments of the embryo / fetus of horses in the first four months of pregnancy. In order to carry out this research, 37 placentas of adult, mixed breed mares were collected and divided into groups at the different stages of development, following the methodology employed by EVANS SACK (1973), until 107 days of gestation, and were subsequently destined for the microscopy of light. The membrane fragments (corium, allantois, amnion and yolk sac) were fixed in 10% formalin and Bouin followed by routine paraffin processing (TOLOSA et al; 2003). Serial slices with a thickness of 5μm were obtained in a microtome (Leica 2165®) which were submitted to staining by the methods of hematoxylin and eosin, picrosirius, Toluidine blue and Masson's trichrome (TOLOSA et al; 2003). In the four groups, the amnion presented with a simple pavement epithelium and mesenchyme without any vascularization, only vascularization was evident when the amnion fused with the allantois, which is also a simple pavement epithelium. The corium presented villi with a low columnar epithelium and brushed edges, uni and binucleated cells, highly vascularized, and the size and quantity of villi differed from the groups. The yolk sac presented an epithelium whose cells were large and globular, resting on the mesenchyme, forming vascular islands full of hemangioblasts, being non-existent in animals from 47 to 107 days of gestation.Item Classificação macroscópica dos dentes de Nasua nasua(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) PIREI, Naira Caroline Godoy; MANÇANARES, Celina Almeida Furlanetto; ROSA, Ricardo Alexandre; CARVALHO, Ana Flávia de; BERTASSOLI, Bruno M.Nasua nasua, also known as coati, is an omnivorous mammal, with wide distribution throughout South America, found from Colombia to Argentina and in Brazil it occupies all regions. The evolution caused in the teeth of mammals multiple variations, adapting the most diverse diets. Due to these variations, it is possible to classify vertebrates by order, gender and family. This work aimed to morphologically classify the teeth of males and females Nasua nausa, in order to classify and compare them with each other and with the morphology of Cannis familiaris (dogs) teeth described in the literature. In this study, five heads of Nasua nasua were used, adults, of both sexes, provided by the scientific breeding CECRIMPAS (UNIFEOB). Two macerated animals had their oral cavity exposed for the photographic documentation of the position of the teeth. In the oral cavity of Nasua nasua due to sexual dimorphism, it was possible to compare the male and female dentition macroscopically. The female has smaller teeth, rounded canines, and the incisor group on the lower part is smaller than on the upper and in the male they are pointed and larger. The animals presented 2x dental formula (I3 / 3, C1 / 1, P4 / 4, M2 / 2), with I = incisors, C = canines, P = premolars and M = molars, in a total of 40 teeth.