10º Encontro Acadêmico de Produção Científica

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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.

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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.

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Author: search.filters.author.FERRAZ, Ana Cláudia Cristiane

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    Membranas embrionárias e fetais de equinos sob análise de microscopia eletrônica de transmissão
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) NASCIMENTO, Priscila Leal do; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luis Rezende; FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Ana Carolina Furlanetto; CARVALHO, Ana Flávia de
    To develop this research, 16 pregnant uteri were used, of which 16 placentas were obtained divided into groups of different stages of development. The material was collected from adult, mixed breed mares up to 100 days of gestation. The membranes were intended for ultra-structural analysis and the results obtained under transmission electron microscopy (MET) were similar to those found by ASSIS-NETO (2005), in bovine membranes, and ITURRIZAGA (2005), in llamas. The fetal membranes did not show relevant structural differences in the different periods studied. The chorion showed columnar epithelium; mesenchymal tissue rich in collagen fibers, the trophoblastic cells being uni, binucleated and rarely trinucleated. A large number of mitochondria were present mainly at the apex of cells. The allantoic presented a simple columnar epithelium with globular nuclei and a large concentration of mitochondria at the cell apex, in the cell base there was a large amount of rough endoplasmic reticulum. The amnion showed a simple, squamous epithelium and at the cellular apex there was a large amount of electrondense granules covered by a rounded halo. The yolk sac epithelium varied from globose to columnar, with uni or binucleate cells in a single layer, with a large amount of rough endoplasmic reticulum, uniformly distributed. Between the endodermal cells, intercellular spaces were found. So far, it is concluded that the mare's placenta is choroidal alloid, epithelial, diffuse, non-deciduous and villous, with corium cells and a yolk sac with characteristics of protein secretion, while amnion presented secretion vesicles, but with less amount of rough endoplasmic reticulum.
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    Estabelecimento do protocolo Agnor para membranas embrionárias e fetais de equinos
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) MANÇANARES, Ana Carolina Furlanetto; MANÇANARES, Celina Almeida Furlanetto; FERRAZ, Ana Cláudia Cristiane; CARVALHO, Ana Flávia de
    The nucleolus organizing regions (RONs) or Nucleolar Organizer Regions (NORs) are slightly stained chromatin regions around which, at the end of the telophase, the nucleolus is formed again after mitosis. A peculiar group of acidic proteins that have a high affinity for silver, are located in the same places as NORs, which gives the same characteristics of being clearly and quickly visualized by stains using silver nitrate. For this study, equine embryo / fetus membranes obtained from 30 placentas were used. Such material was collected from adult, mixed breed mares and at different gestation periods of up to 100 days. Fragments of the embryo / fetus membranes were fixed in 10% formaldehyde for routine histological procedures (TOLOSA et al, 2003). Serial cuts with a thickness of 5μm were obtained in a microtome (Leica 2165®,) which were dewaxed and immersed in a final solution 1: 2, consisting of colloidal solution (gelatin 2% and formic acid 1%) and silver nitrate solution (AgNO3) (1: 1). They were taken to the oven at 37 ° C, in a humid chamber, for approximately 20 minutes. After washing the slides with water, a 1% sodium thiosulfate solution was applied for one minute and followed by washing and drying at room temperature, being stored in a darkroom. After the silver impregnation staining of the NORs (Nucleolar Organizer Regions) some slides were subjected to Eosin baths so that the cytoplasm was stained and thus obtaining easier cell individualization. The results obtained were appropriate, showing the appropriate protocol, and the eosin background provided a better visualization of cell delimitations, thus facilitating the possible counting of NORs per cell.
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    Análise macroscópica dos anexos embrionários e fetais de equinos em até 107 dias de gestação
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de
    The horse (Equus caballus, Linneaus 1758) is a large ungulate mammal of the Equidea family, one of the seven modern species of the Equus genus. Domesticated for thousands of years, it is an unparalleled animal, of unusual beauty, grace, sensitivity and athletic ability; a fascinating animal for study, as described by Evans et al. (nineteen ninety); Ginther (1992) and Dantzer et al. (1998). Because it is not a simple model, it offers a comprehensive range for research and challenges scientists. Equine placentation and its embryonic development are some of the issues to be explored. Thus, this study aimed to describe microscopically the embryonic attachments of the embryo / fetus of horses in the first four months of pregnancy. In order to carry out this research, 37 placentas of adult, mixed breed mares were collected and divided into groups at the different stages of development, following the methodology employed by EVANS SACK (1973), until 107 days of gestation, and were subsequently destined for the microscopy of light. The membrane fragments (corium, allantois, amnion and yolk sac) were fixed in 10% formalin and Bouin followed by routine paraffin processing (TOLOSA et al; 2003). Serial slices with a thickness of 5μm were obtained in a microtome (Leica 2165®) which were submitted to staining by the methods of hematoxylin and eosin, picrosirius, Toluidine blue and Masson's trichrome (TOLOSA et al; 2003). In the four groups, the amnion presented with a simple pavement epithelium and mesenchyme without any vascularization, only vascularization was evident when the amnion fused with the allantois, which is also a simple pavement epithelium. The corium presented villi with a low columnar epithelium and brushed edges, uni and binucleated cells, highly vascularized, and the size and quantity of villi differed from the groups. The yolk sac presented an epithelium whose cells were large and globular, resting on the mesenchyme, forming vascular islands full of hemangioblasts, being non-existent in animals from 47 to 107 days of gestation.
Direito autoral ou direito de autor é um conjunto de prerrogativas conferidas por lei à pessoa física ou jurídica criadora da obra intelectual, para que ela possa usufruir de quaisquer benefícios morais e patrimoniais resultantes da exploração de suas criações É derivado dos direitos individuais e situa-se como um elemento híbrido, especial e autônomo dentro do direito civil.