8º Encontro Acadêmico de Produção Científica
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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Item Influência do estresse causado pela tosquia e aumento de peso ao nascer de cordeiros(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; FRANCIOLLI, André Luis Rezende; FAVARON, Phelipe Oliveira; WENCESLAU, Cristiane Valverde; RIBEIRO, Luiz Alberto Oliveira; MANÇANARES, Celina de Almeida Furlanetto; MIGLINO, Maria Angélica; BOMBONATO, Pedro PrimoReproductive losses in the southern region of Brazil have been a major problem for sheep farmers and studies in the area report that the weight of lambs at birth when greater than 4Kg allows it to survive during the winter period in which they are born. Due to the need for greater weight at birth, to ensure greater survival, numerous procedures have been performed in an attempt to promote lower mortality. The purpose of this work was to verify the influence of shearing on transplacental glycogen transport and its supposed relationship with the increase in birth weight in lambs (Ovies aries). For the realization of the project 9 sheep of the Australian Merino breed were used which were raised at the Federal University of Rio Grande do Sul - Porto Alegre / RS) - UFRGS in collaboration with the Lutheran Brazilian University - ULBRA. The animals were divided into 2 groups: the first with 5 sheared animals (OVE) at 70 days of the perinatal period and the second with four animals that were kept as control (OVC). The fetuses were collected through a surgical cesarean section performed at the end of pregnancy. For comparative purposes, placental and fetal weight were initially measured; to characterize the placenta, the number of placentons and the length, width and thickness of the placentonium were quantified, with the aid of a caliper. The collected placental material was fixed, dehydrated, diaphanized and included in paraffin, for later making slides and quantifying glycogen. As a partial macroscopic result, the placentas of the sheep in the sheared group had a weight 13% greater than those in the control group, in the fetuses the difference was more expressive, the weight of the fetuses sampled in the sheared group was 25% greater than those in the second group. The differences between the measures of thickness, length and width were insignificant, but always with higher rates in the tested group.Item Estudo da pluripotencialidade do fígado fetal canino nos diferentes períodos gestacionais(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) VIEIRA, Eduardo; MARTINS, Daniele dos Santos; WENCESLAU Cristiane Valverde; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de; ROSA, Ricardo Alexandre; MIGLINO, Maria AngélicaThe study of the differentiation capacity of some cells, called stem cells or '' sistem '' cells represents an important tool for understanding and developing new research, as well as an enormous potential for discovering the treatment of numerous diseases. These embryonic stem cells are pluripotent, capable of multiplication and differentiation, and can proliferate in several cell types depending on the culture conditions. The identification of factors that lead to the direction of the cell differentiation process will allow, from embryonic stem cells, the culture to be carried out in a controlled manner among the most different cell types, making bioengineering possible. In this work, cells of the canine fetal liver were studied. The liver performs an important hematopoietic function during fetal development, while in the adult it has a multiplicity of functions such as: bile excretion, storage, synthesis and biotransformation. The livers, for study, were obtained from fetuses with gestational age with an average of 25, 35, 45 and 55 days, from females of mixed breed dogs that underwent ovariosalpingohesterectomy. These canine fetal liver cells were analyzed histologically to characterize cell differentiation, using previously established histological methods.Item Características de células fibroblast-like derivadas do cultivo do saco vitelino em diferentes períodos gestacionais(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) WENCESLAU, Cristiane Valverde; MARTINS, Daniele dos Santos; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luiz Resende; AGRESTE, Fernanda R.; MORINI, Adriana Caroprezo; MORINI JÚNIOR, João Carlos; CARVALHO, Ana Flávia de; MIGLINO, Maria AngélicaBased on the knowledge regarding the erythropoietic activity of the vitelline membrane, which is considered the first hematopoietic and vascular site that appears in mammals during organogenesis, added to the erythropoiesis support function performed by mesenchymal stem cells, the present work aims to verify the cell expansion of the yolk sac using the explant technique, in view of the cell niches found in the fetal annex. For the cultivation of the yolk sac, 5 bitches with gestational ages of 30, 40 and 60 days were used, and were checked by ultrasound and Crow - Rump of each fetus according to Evans & Sack (1979). The bitches were submitted to ovariosalpingohisterectomy to collect the fetal attachment. All material was collected at the Faculty of Veterinary Medicine and Animal Science at the University of São Paulo - USP. The yolk sac was fragmented manually (mechanical fragmentation), with the aid of a scalpel blade until obtaining a homogenate of tissue fragments and cell groups, which were implanted in the 5 ml culture bottle with the modified Eagle Dulbeco medium - DMEM High with 10% fetal serum, streptomycin and penicillin. The cells were incubated at 38.5 degrees Celsius, at 100% humidity and 5% CO 2. The morphological description of the cell culture was performed periodically using the Nikon TS100 phase contrast microscope. The cells maintained in these conditions grew in the form of monolayer, with different fusiform cell types and large cells with cytoplasmic extensions like fibroblast-like.Item Análise macroscópica do aparelho reprodutor masculino do ouriço cacheiro(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) CASALS, Juliana Barbosa; BARREIRO, Juliana Regina; ROSA, Ricardo Alexandre; MANÇANARES, Celina Almeida Furlanetto; FAVARON, Phelipe Oliveira; CARVALHO, Ana Flávia de; MIGLINO, Maria Angélica; MARQUES, Leandro de Oliveira; ANDRADE, Filipe Ian Bindez de; NASCIMENTO, Priscila Leal do; FERRAZ, Ana Cláudia Cristiane; AMBRÓSIO, Carlos EduardoRodents comprise most of the mammalian class. There are currently 2,021 species, organized into 443 genera and 29 families. Hedgehogs (Coendou villosus), are animals that in captivity, reproduce with some ease and become tame. In the urban and metropolitan region their presence is becoming common and this can be identified by the number of accidents involving dogs in the yard, registered in veterinary clinics. This work aimed to describe macroscopically the reproductive apparatus of Coendou villosus (hedgehog) to better clarify the anatomy of this important organ in the wild rodent model, since very little is known about this species. We compared the results obtained with other rodent species already studied. For the macroscopic description of the male reproductive system, 2 animals from donations were used, these animals ended up dying after attack by dogs.Item Estudo histológico da glândula pineal de Nasua nasua (Quati) empregando microscopia de luz(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-11-30) FAVARON, Phelipe Oliveira; MANÇANARES, Celina Almeida Furlanetto; AMBRÓSIO, Carlos Eduardo; CASALS, Juliana Barbosa; MIGLINO, Maria Angélica; CARVALHO, Ana Flávia deNasua nasua or coati is a mammal of the carnivorous order and Procyonidae family. They are animals that live in flocks and have a wide geographical distribution throughout South America. The pineal gland is an endocrine gland, whose melatonin synthesis is influenced by the light and dark periods of the day. The objective of this work was to describe the histology of the coati pineal gland, evaluating its arrangement and cellular composition. For this work, 5 pineal glands were used, which were processed according to routine tissue processing techniques and then a paraffin was included. The pineal gland microscopically has a shape similar to a small pine cone and is divided into 3 parts: apex, body and base. The predominant cells in the glandular parenchyma were pinealocytes, which have large, rounded nuclei with little apparent cytoplasm. Externally the coati pineal gland is lined by a connective tissue capsule from which septa depart into the gland. Secretions were observed at the glandular apex.Item Caracterização da morfologia externa de fetos de Paca(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) FRANCIOLLI, André Luis Rezende; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele dos Santos; MANÇANARES, Celina Almeida Furlanetto; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde; COSTA, Gerlane M.; MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; MIGLINO, Maria AngélicaThe present scientific investigation aimed to study the embryonic development of pacas (Agouti Paca), with a view to the external morphological description of fetuses. Five fetuses were used, three of which were donated from the donation maintained by the Faculty of Agricultural and Veterinary Sciences of Jaboticabal - UNESP and two donated from the collection of biological material from FMVZ - USP. Fetuses 1 and 2 with 5.7 and 6.8 cm of Crow Rump, respectively, presented as morphological characteristics: marked facial immaturity, with the eyes covered by a prominent lens; the earlobes had a low level of development. The mouth had a small opening, where the formation of the incisor teeth was observed. Fetuses 3 and 4 with 9.6 and 10.5 cm of Crow Rump, respectively, showed well-developed ears, thoracic and pelvic limbs were in an equal degree of development with the presence of claws and development of facial features, like vibrissae around the nasal edges, the eyes remain protected by a prominent lens and the mouth contained the incisor teeth in an intermediate stage of development. Fetus 5, with 15.5 cm of Crow Rump, presented as a striking feature, the body covered by hair, thoracic and pelvic limbs in an equal degree of development with claws, development of facial characteristics, such as vibrissae, prominent eyes and well-developed ears. In all fetuses, the genital tubercle and the anal rim can be seen in the perineal region.