8º Encontro Acadêmico de Produção Científica
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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A produção do conhecimento é o principal recurso de que dispõe a sociedade para criar oportunidades de crescimento individual e coletivo, e o desenvolvimento das organizações está ligado à disponibilidade de recursos tecnológicos e humanos adequados a seus propósitos.
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Item Avaliação da adaptação de ovelhas ao manejo de ordenha mecânica através do comportamento(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) NABI Mariana Sintoni; BOCHINI, João Carlos; DEFAVARI JÚNIOR, Antônio Carlos; OLIVEIRA JÚNIOR, Mauro; HEBLING, Mariana R.; HUCKE, Erica Elgelberg Teixeira da SilvaThe study of animal behavior is growing more and more due to the concern that exists in several countries with the preservation of animal welfare in general, and especially in production systems, as the conditions of animal breeding reflect directly on the quality of products generated by them. The relationship between animal welfare and animal production, in particular milk has been highlighted in recent studies, and the evaluation of animal welfare in milk production systems focuses on the quality of life of these farm animals and consequently the quality of the milk produced, taking into account economic, ethnic, social and environmental factors. In this way, the present research project intends to reveal the importance of behavioral studies, using sheep as an experimental model, with the objective of evaluating their behavior when subjected to mechanical milking management, electing possible indicators of well-being in these conditions as well as the interference of milking management under the maternal filial relationship.Item Técnica para cateterização do espaço subaracnóideo em ovinos(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) BOCHINI, João Carlos; ALVES, Jeferson Douglas Soares; SILVA, Angélica do Rocio Carvalho; NABI, Mariana Sintoni; DEFAVARI JÚNIOR, Antônio Carlos; FELICIO, Luciano Freitas; PANATTONI, João Flávio; HUCKE, Érica Engelberg Teixeira da SilvaTechniques for collecting cerebrospinal fluid (CSF) in some animal species have been used for some time. However, the existing techniques present restrictions, both methodological and for animal welfare, which need to be minimized. In this way, 6 multiparous sheep (Ovis aries) of the Santa Inês breed were used to obtain CSF through a puncture of the subarachnoid space with the aid of an epidural catheter between the cervical vertebrae C1 and C2 fixed on the epidermis. After anesthetic recovery of the animals, CSF was harvested for 5 consecutive days. 3 ml of CSF were obtained per animal / day. Throughout the experimental period, the sheep did not show neurological changes, nor a sign of infection, as well as no changes in the CSF's physical characteristics. We conclude that the method for catheterization of the subarachnoid space presented above is feasible and effective for obtaining continuous CSF for prolonged periods, thus avoiding more invasive methods with the same objective.Item Leucemia canina: casos diagnosticados no período de 2003 a 2006(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) SALA, Rodrigo V.; DAVANSO, André L.; SANCHES, Carolina D. C.; ARAÚJO, Luciana R.; BAILOME, Mayara C.; SIQUEIRA, Geraldo P.; BANDARRA, Márcio B.; DI SANTIS, Giovana W.; THOMÉ, Helder E.; MOURA, Veridiana M. B. D. de; BANDARRA, Enio P.Leukemia is characterized by the presence of malignant neoplastic cells of hemolymphatic origin in the blood and / or bone marrow. They can be classified into lymphoblastic or myeloblastic, being divided into acute or chronic according to the predominant cell characteristic and subclassified according to the cell type found. As a definitive diagnosis, in addition to histopathological examination, other techniques such as immunohistochemistry, enzymatic cytochemistry and immunophenotyping can be used, which are more specific and effective for the classification of leukemias. Approximately 30% of canine leukemias are lymphoid. This study aimed to survey the cases of leukemia in dogs diagnosed by the Pathology Laboratory of the Faculty of Veterinary Medicine of São João da Boa Vista -SP - UNIFEOB, in the period from 2003 to 2006, totaling 10 cases of leukemia of origin lymphoid and three cases presenting foci of lymphadenization.Item Avaliação do ganho de peso diário de cordeiros segundo o manejo de desmame(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) DEFAVARI JÚNIOR, Antônio Carlos; BOCHINI, João Carlos; NABI, Mariana Sintoni; PANATTONI, João Flávio; BALIEIRO, Cristiano Carvalho; HUCKE, Erica Elgelberg Teixeira da SilvaStudies related to animal production and reproduction aim to minimize economic losses, since the number of lambs born and weaned per female / year is directly related to the productive efficiency of the herd. In this way, the greater the amount of lambs born and weaned by sheep, the greater number of animals for sale, for replacement of matrices and for the selection of the flock. The present study aimed to evaluate lambs' weight gain in three different types of weaning management, which were: the precocious, the mixed system and exclusive breastfeeding. Among the types of management mentioned in the experiment, the mixed management group was the one that demonstrated greater advantages in relation to the others.Item Influência do estresse causado pela tosquia e aumento de peso ao nascer de cordeiros(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; FRANCIOLLI, André Luis Rezende; FAVARON, Phelipe Oliveira; WENCESLAU, Cristiane Valverde; RIBEIRO, Luiz Alberto Oliveira; MANÇANARES, Celina de Almeida Furlanetto; MIGLINO, Maria Angélica; BOMBONATO, Pedro PrimoReproductive losses in the southern region of Brazil have been a major problem for sheep farmers and studies in the area report that the weight of lambs at birth when greater than 4Kg allows it to survive during the winter period in which they are born. Due to the need for greater weight at birth, to ensure greater survival, numerous procedures have been performed in an attempt to promote lower mortality. The purpose of this work was to verify the influence of shearing on transplacental glycogen transport and its supposed relationship with the increase in birth weight in lambs (Ovies aries). For the realization of the project 9 sheep of the Australian Merino breed were used which were raised at the Federal University of Rio Grande do Sul - Porto Alegre / RS) - UFRGS in collaboration with the Lutheran Brazilian University - ULBRA. The animals were divided into 2 groups: the first with 5 sheared animals (OVE) at 70 days of the perinatal period and the second with four animals that were kept as control (OVC). The fetuses were collected through a surgical cesarean section performed at the end of pregnancy. For comparative purposes, placental and fetal weight were initially measured; to characterize the placenta, the number of placentons and the length, width and thickness of the placentonium were quantified, with the aid of a caliper. The collected placental material was fixed, dehydrated, diaphanized and included in paraffin, for later making slides and quantifying glycogen. As a partial macroscopic result, the placentas of the sheep in the sheared group had a weight 13% greater than those in the control group, in the fetuses the difference was more expressive, the weight of the fetuses sampled in the sheared group was 25% greater than those in the second group. The differences between the measures of thickness, length and width were insignificant, but always with higher rates in the tested group.Item Estudo da pluripotencialidade do fígado fetal canino nos diferentes períodos gestacionais(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) VIEIRA, Eduardo; MARTINS, Daniele dos Santos; WENCESLAU Cristiane Valverde; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de; ROSA, Ricardo Alexandre; MIGLINO, Maria AngélicaThe study of the differentiation capacity of some cells, called stem cells or '' sistem '' cells represents an important tool for understanding and developing new research, as well as an enormous potential for discovering the treatment of numerous diseases. These embryonic stem cells are pluripotent, capable of multiplication and differentiation, and can proliferate in several cell types depending on the culture conditions. The identification of factors that lead to the direction of the cell differentiation process will allow, from embryonic stem cells, the culture to be carried out in a controlled manner among the most different cell types, making bioengineering possible. In this work, cells of the canine fetal liver were studied. The liver performs an important hematopoietic function during fetal development, while in the adult it has a multiplicity of functions such as: bile excretion, storage, synthesis and biotransformation. The livers, for study, were obtained from fetuses with gestational age with an average of 25, 35, 45 and 55 days, from females of mixed breed dogs that underwent ovariosalpingohesterectomy. These canine fetal liver cells were analyzed histologically to characterize cell differentiation, using previously established histological methods.Item Suspeita da produção de b- lactamase de espectro estendido (ESBL) enterobactérias isoladas em caso de mastite ambiental(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) REIS, Carolina Barbosa Malek dos; TURRI, Rosimary de Jesus Gomes; ORIANI, Maria Raquel de GodoyThe great difficulty in controlling mastitis is that the indiscriminate use of antibiotics can generate multiresistant microorganisms. The literature describes that enterobacteria are producers of extended-spectrum b-lactamase enzymes (ESBL) which leads to resistance to b-lactam antibiotics. The objective of this work was to isolate ESBL-pro-producing enterobacteria from bovine mastitic milk. The technique used in the antibiogram was the disk diffusion method recommended by the NCCLS (2002). Strains of Escherichia coli, Citrobacter, Protheus, Shigella, Enterobacter, Salmonella and Klebsiella were obtained, suggestive of producing ESBL. It is concluded that the presence of multiresistant strains is due to the inappropriate use of antibiotics on the property.Item Características de células fibroblast-like derivadas do cultivo do saco vitelino em diferentes períodos gestacionais(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) WENCESLAU, Cristiane Valverde; MARTINS, Daniele dos Santos; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luiz Resende; AGRESTE, Fernanda R.; MORINI, Adriana Caroprezo; MORINI JÚNIOR, João Carlos; CARVALHO, Ana Flávia de; MIGLINO, Maria AngélicaBased on the knowledge regarding the erythropoietic activity of the vitelline membrane, which is considered the first hematopoietic and vascular site that appears in mammals during organogenesis, added to the erythropoiesis support function performed by mesenchymal stem cells, the present work aims to verify the cell expansion of the yolk sac using the explant technique, in view of the cell niches found in the fetal annex. For the cultivation of the yolk sac, 5 bitches with gestational ages of 30, 40 and 60 days were used, and were checked by ultrasound and Crow - Rump of each fetus according to Evans & Sack (1979). The bitches were submitted to ovariosalpingohisterectomy to collect the fetal attachment. All material was collected at the Faculty of Veterinary Medicine and Animal Science at the University of São Paulo - USP. The yolk sac was fragmented manually (mechanical fragmentation), with the aid of a scalpel blade until obtaining a homogenate of tissue fragments and cell groups, which were implanted in the 5 ml culture bottle with the modified Eagle Dulbeco medium - DMEM High with 10% fetal serum, streptomycin and penicillin. The cells were incubated at 38.5 degrees Celsius, at 100% humidity and 5% CO 2. The morphological description of the cell culture was performed periodically using the Nikon TS100 phase contrast microscope. The cells maintained in these conditions grew in the form of monolayer, with different fusiform cell types and large cells with cytoplasmic extensions like fibroblast-like.Item Análise macroscópica do aparelho reprodutor masculino do ouriço cacheiro(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) CASALS, Juliana Barbosa; BARREIRO, Juliana Regina; ROSA, Ricardo Alexandre; MANÇANARES, Celina Almeida Furlanetto; FAVARON, Phelipe Oliveira; CARVALHO, Ana Flávia de; MIGLINO, Maria Angélica; MARQUES, Leandro de Oliveira; ANDRADE, Filipe Ian Bindez de; NASCIMENTO, Priscila Leal do; FERRAZ, Ana Cláudia Cristiane; AMBRÓSIO, Carlos EduardoRodents comprise most of the mammalian class. There are currently 2,021 species, organized into 443 genera and 29 families. Hedgehogs (Coendou villosus), are animals that in captivity, reproduce with some ease and become tame. In the urban and metropolitan region their presence is becoming common and this can be identified by the number of accidents involving dogs in the yard, registered in veterinary clinics. This work aimed to describe macroscopically the reproductive apparatus of Coendou villosus (hedgehog) to better clarify the anatomy of this important organ in the wild rodent model, since very little is known about this species. We compared the results obtained with other rodent species already studied. For the macroscopic description of the male reproductive system, 2 animals from donations were used, these animals ended up dying after attack by dogs.Item Análise comparativa do número de corpos de neurônios em áreas do córtex cerebral de diferentes raças de cães(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-11-30) ESTEVES, A.; PRADA, I. L.S.; MANÇANARES, C. A. F.; ROSA, R. A.; MIGLINO, M. A.; PAFFARO, A. M. A.; PAFFARO JÚNIOR, V. A.; CARVALHO, A. F. de.In this study, three dog breeds were focused, including Boxer, Doberman and Rotwailler, with constitutional types and functional characteristics that are distinct and distinct from each other. From the 4 brains of each race, totaling 16 fragments were removed from some areas of the cerebral cortex (auditory, visual, somesthetic, motor, limbic, olfactory and taste) that were prepared according to conventional histological technique and stained with modified cresyl violet. Through the manual - visual counting of the average number of neuron bodies, comparative data were sought between these brain areas versus races associating constitutional types versus functional aptitude. The slides were analyzed with the aid of the Zeiss O Axioscope, coupled with the KS-400 image analysis program version 2.0 Kontron - Zeiss O. The results allow to conclude that the quantitative distribution of neurons in the different cortical areas studied shows significant differences that establish correlations with the functional aptitudes and respective constitutional types of the focused races.