Browsing by Author "WENCESLAU, Cristiane Valverde"

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Análise morfológica das células do aspirado medular de fetos caninos
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) MÁRIO, Lara Carolina; BORGHESI, Jéssica; MELLO, Ana Carolina Landentin; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde
The adult bone marrow is a source of stem cells (CT), which include the hematopoietic stem cells (HSC) that originate the entire definitive hematopoietic lineage; and mesenchymal stem cells (MSC), and or stromal which are responsible for providing a favorable environment for hematopoiesis. In addition to these main cell types, bone marrow is composed of several parents, differentiated cells and an extracellular matrix. This environment where CT is found is called a niche, as it houses a large amount of CT and cellular progenitors that are kept quiescent, acting normally and or in the face of injuries. However, little is known about the fetal bone marrow niche. It is known that the bone marrow niche is derived during the ontogenesis of the yolk sac, which subsequently migrates to various hematopoietic organs such as the placenta and liver, ultimately settling in the bone marrow. Thus, the present work aims to describe morphologically the cell types of canine fetal bone marrow at 40 and 60 days of gestation. For this purpose, 6 pregnant female uteri were used to collect the medullary aspirate from the femur bone and later structural and ultrastructural morphological analysis. The results demonstrated that the canine fetal bone marrow at 60 days of gestation has cell types similar to that of the adult bone marrow as well as CT, thus being able to be used as a source of CT isolation.
Anatomia microscópica do sistema reprodutor feminino do Tamanduá Bandeira
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) GARCIA, Rafael de Carvalho; BRANCO, Èrika Renata; LIMA, Ana Rita de; OLIO, Rennan Lopes; WENCESLAU, Cristiane Valverde; MANÇANARES, Celina Almeida Furlanetto; THOMAZ, Juliana Mantovani; CARVALHO, Ana Flávia de
The giant anteater is a large animal and can be very aggressive with its claws if disturbed, it belongs to the Xenarthra Order, Family Myrmecophagidae, which are toothless members, and therefore called edentats. Some reports have been made about this species, but many relevant data have not been published, emphasizing basic concepts of morphology and biometrics, which could be extremely useful for the development of reproductive biotechnologies, aiming at the preservation of the species. For this study, two animals donated by UFRA – PA were used, in which the microscopic characteristics of the female reproductive system were analyzed, in order to clarify the peculiar aspects of the species. The results obtained reveal that the ovaries, fallopian tubes and uterus are similar histologically to those of domestic animals.
Características de células fibroblast-like derivadas do cultivo do saco vitelino em diferentes períodos gestacionais
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) WENCESLAU, Cristiane Valverde; MARTINS, Daniele dos Santos; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luiz Resende; AGRESTE, Fernanda R.; MORINI, Adriana Caroprezo; MORINI JÚNIOR, João Carlos; CARVALHO, Ana Flávia de; MIGLINO, Maria Angélica
Based on the knowledge regarding the erythropoietic activity of the vitelline membrane, which is considered the first hematopoietic and vascular site that appears in mammals during organogenesis, added to the erythropoiesis support function performed by mesenchymal stem cells, the present work aims to verify the cell expansion of the yolk sac using the explant technique, in view of the cell niches found in the fetal annex. For the cultivation of the yolk sac, 5 bitches with gestational ages of 30, 40 and 60 days were used, and were checked by ultrasound and Crow - Rump of each fetus according to Evans & Sack (1979). The bitches were submitted to ovariosalpingohisterectomy to collect the fetal attachment. All material was collected at the Faculty of Veterinary Medicine and Animal Science at the University of São Paulo - USP. The yolk sac was fragmented manually (mechanical fragmentation), with the aid of a scalpel blade until obtaining a homogenate of tissue fragments and cell groups, which were implanted in the 5 ml culture bottle with the modified Eagle Dulbeco medium - DMEM High with 10% fetal serum, streptomycin and penicillin. The cells were incubated at 38.5 degrees Celsius, at 100% humidity and 5% CO 2. The morphological description of the cell culture was performed periodically using the Nikon TS100 phase contrast microscope. The cells maintained in these conditions grew in the form of monolayer, with different fusiform cell types and large cells with cytoplasmic extensions like fibroblast-like.
Caracterização da morfologia externa de fetos de Paca
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) FRANCIOLLI, André Luis Rezende; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele dos Santos; MANÇANARES, Celina Almeida Furlanetto; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde; COSTA, Gerlane M.; MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; MIGLINO, Maria Angélica
The present scientific investigation aimed to study the embryonic development of pacas (Agouti Paca), with a view to the external morphological description of fetuses. Five fetuses were used, three of which were donated from the donation maintained by the Faculty of Agricultural and Veterinary Sciences of Jaboticabal - UNESP and two donated from the collection of biological material from FMVZ - USP. Fetuses 1 and 2 with 5.7 and 6.8 cm of Crow Rump, respectively, presented as morphological characteristics: marked facial immaturity, with the eyes covered by a prominent lens; the earlobes had a low level of development. The mouth had a small opening, where the formation of the incisor teeth was observed. Fetuses 3 and 4 with 9.6 and 10.5 cm of Crow Rump, respectively, showed well-developed ears, thoracic and pelvic limbs were in an equal degree of development with the presence of claws and development of facial features, like vibrissae around the nasal edges, the eyes remain protected by a prominent lens and the mouth contained the incisor teeth in an intermediate stage of development. Fetus 5, with 15.5 cm of Crow Rump, presented as a striking feature, the body covered by hair, thoracic and pelvic limbs in an equal degree of development with claws, development of facial characteristics, such as vibrissae, prominent eyes and well-developed ears. In all fetuses, the genital tubercle and the anal rim can be seen in the perineal region.
Descrição microscópica do sistema reprodutor feminino do Tamanduá Mirim
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) OLIO, Renan Lopes; LOBO, Luis Miguel; WENCESLAU, Cristiane Valverde; MANÇANARES, Celina Almeida Furlanetto; BRANCO, Érika Renata; GARCIA, Rafael de Carvalho; LIMA, Ana Rita de; CARVALHO, Ana Flávia de
The juvenile anteater is an extremely captivating and docile animal, belongs to the Xenarthra Order, Family Myrmecophagidae, which are toothless members, and therefore called edentats. As it is an animal of our fauna, data about the species, with regard to morphology and physiological aspects, are very scarce, and generally focus on diseases and external parasites, from capture and expeditions. For this study, two animals donated by UFRA – PA were used, in which the macroscopic characteristics of the female reproductive system were analyzed, in order to clarify the morphological aspects of the female reproductive system, peculiar to the species, in order to assist in their preservation and create reproductive biotechnology tools. The obtained results reveal that the ovaries are longilinear, the uterine tubes tortuous, and the simple uterus in pear shape, as well as in the sloth, in the woman and in the primates. The cervix is quite thick and the vagina communicates with the vestibule and the vulva.
Estabelecimento das condições de isolamento e cultivo das células tronco mesenquimais da médula óssea em fetos de cães
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) BORGHESI, Jéssica; MARIO, Lara Carolina; MELLO, Ana Carolina Landentin; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde
Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into multiple differentiated terminal strains, including bone, cartilage, muscle, nerve, adipose and marrow stromal cells in vivo and in vitro. These cells are in addition to being isolated from the adult bone marrow and can be isolated from the fetal bone marrow. However, there is little study regarding the isolation technique of MSCs from fetal bone marrow. In this way, the work proposed to isolate MSC from canine fetuses based on the established methodology for obtaining MSC from adult human bone marrow. For this purpose, 12 pregnant female uteri were used to collect the medullary aspirate from the femur bone. The culture medium of choice was alpha-MEM and 15% fetal bovine serum, which provided the isolation of positive CTM-CD44.
Influência do estresse causado pela tosquia e aumento de peso ao nascer de cordeiros
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; FRANCIOLLI, André Luis Rezende; FAVARON, Phelipe Oliveira; WENCESLAU, Cristiane Valverde; RIBEIRO, Luiz Alberto Oliveira; MANÇANARES, Celina de Almeida Furlanetto; MIGLINO, Maria Angélica; BOMBONATO, Pedro Primo
Reproductive losses in the southern region of Brazil have been a major problem for sheep farmers and studies in the area report that the weight of lambs at birth when greater than 4Kg allows it to survive during the winter period in which they are born. Due to the need for greater weight at birth, to ensure greater survival, numerous procedures have been performed in an attempt to promote lower mortality. The purpose of this work was to verify the influence of shearing on transplacental glycogen transport and its supposed relationship with the increase in birth weight in lambs (Ovies aries). For the realization of the project 9 sheep of the Australian Merino breed were used which were raised at the Federal University of Rio Grande do Sul - Porto Alegre / RS) - UFRGS in collaboration with the Lutheran Brazilian University - ULBRA. The animals were divided into 2 groups: the first with 5 sheared animals (OVE) at 70 days of the perinatal period and the second with four animals that were kept as control (OVC). The fetuses were collected through a surgical cesarean section performed at the end of pregnancy. For comparative purposes, placental and fetal weight were initially measured; to characterize the placenta, the number of placentons and the length, width and thickness of the placentonium were quantified, with the aid of a caliper. The collected placental material was fixed, dehydrated, diaphanized and included in paraffin, for later making slides and quantifying glycogen. As a partial macroscopic result, the placentas of the sheep in the sheared group had a weight 13% greater than those in the control group, in the fetuses the difference was more expressive, the weight of the fetuses sampled in the sheared group was 25% greater than those in the second group. The differences between the measures of thickness, length and width were insignificant, but always with higher rates in the tested group.