Browsing by Author "SANTOS, Thainá de Oliveira"

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    Análise morfológica de embriões ovinos produzidos em laboratório a partir de oócitos aspirados de doadoras multíparas, primíparas e pré - púberes da raça Santa Inês
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) MARTINS, João Flávio Panattoni; SANTOS, Thainá de Oliveira; SILVA, Marcos Donizete da; SOUZA, Bruna Manzer de; OLIVEIRA, Priscila C. de; ROSAS FILHO, Adauto de Carvalho; ABREU, Rogério Navarro de; FIGUEIREDO, Cristiane Leite
    This work aimed to analyze the morphology of embryos at seven days of development, produced in the laboratory from oocytes aspirated from three different classes of donors of the Santa Inês race (multiparous, primiparous and prepubertal). The analyzed embryos had a discoid shape and were delimited by the pellucid zone (ZP). In the morula and initial blastocyst stages, the ZP measured from 5 to 10 μm in thickness, revealing to be thinner in the expanded blastocyst phase. In general, the structure and ultrastructure of Santa Inês sheep embryos, originating from in vitro fertilization (IVF) and evaluated in this study, corroborate many of the observations by Colarco and Mclaren (1976), Ferrer et al. (1995) and Coccero et al. (2002). The most striking aspect was the intense uniform distribution of lipid inclusions in the cytoplasm of the blastomeres, both in the internal cell mass and in the trophoblastic cells, regardless of the origin of the oocyte, suggesting that the morphology seems to be conditioned mainly to the culture system employed, which seems to favor the accumulation of intracellular lipids.
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    Estudo de embriões ovinos em laboratório a partir de doadoras juvenis da raça Santa Inês submetidas a estimulação ovariana
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2010-05-30) MARTINS, João Flávio Panattoni; SANTOS, Thainá de Oliveira; SILVA, Marcos Donizete da; SOUZA, Bruna Nanzer; OLIVEIRA, Priscila Carvalho de; FIGUEIREDO, Cristiane Leite; ROSAS FILHO, Adauto de Carvalho; ABREU, Rogério Navarro de
    This study aimed to verify the superovulatory response of juvenile Santa Inês sheep and to evaluate the viability of oocytes obtained for in vitro production (IVP) of embryos. Eight females aged between 6 and 8 weeks were selected, distributed in 2 homogeneous groups of 04 animals. The females in Group 1 (G1) were submitted to exogenous ovarian stimulation and in Group 2 (G2) the females were aspirated free of hormonal treatment. From a total of 309 retrieved oocytes (G1 = 187 and G2 = 122), cleavage rates of 49.2% and 40.2% were obtained for G1 (n = 92) and G2 (n = 45) respectively, and 18.72% and 17.96% of viable embryo development in D7 (G1 = 35 and G2 = 19). Although the results suggest a better efficiency in the recovery of oocytes and cleavage rate in G1, the rates of obtaining viable embryos in D7 did not differ numerically between the two groups. Our results suggest that, although there was great variation in the amount of oocytes obtained and the production of embryos among animals, regardless of treatment, the production of embryos by in vitro fertilization (IVF) from juvenile females treated or not with exogenous hormones proved to be viable, even with good efficiency of the hormonal protocol used for females of the Santa Inês breed.