Browsing by Author "ORIANI, Maria Raquel de Godoy"

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    Influência da concentração de antígeno na síntese de anticorpos de resposta primária e secundária em animais de laboratório
    (Fundação de Ensino Octávio Bastos, 2004-09-30) ARAÚJO, Fernando A. de; JORGE NETO, Pedro N.; PITTA, Rodrigo de M.; JORGE, Rômulo da S.; ORIANI, Maria Raquel de Godoy; TURRI, Rosimary de J. G.
    Study carried out to investigate primary and secondary immune response in mice, using 1%, 2.5% and 4% sheep red blood cell solution as antigen. The animals received two doses of antigens at an interval of one week when bleeding was then performed to obtain serums of primary and secondary immune response. Serum titration was performed using the microhemagglutination technique with and without 2 mercaptoethanol, to verify the concentrations of IgM and IgG. According to the obtained titles, it was concluded that the best concentration of antigens for the induction of antibody synthesis was 2.5%, using the lowest concentration a satisfactory response was not obtained and that of 4% induced the death of several animals.
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    Suspeita da produção de b- lactamase de espectro estendido (ESBL) enterobactérias isoladas em caso de mastite ambiental
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) REIS, Carolina Barbosa Malek dos; TURRI, Rosimary de Jesus Gomes; ORIANI, Maria Raquel de Godoy
    The great difficulty in controlling mastitis is that the indiscriminate use of antibiotics can generate multiresistant microorganisms. The literature describes that enterobacteria are producers of extended-spectrum b-lactamase enzymes (ESBL) which leads to resistance to b-lactam antibiotics. The objective of this work was to isolate ESBL-pro-producing enterobacteria from bovine mastitic milk. The technique used in the antibiogram was the disk diffusion method recommended by the NCCLS (2002). Strains of Escherichia coli, Citrobacter, Protheus, Shigella, Enterobacter, Salmonella and Klebsiella were obtained, suggestive of producing ESBL. It is concluded that the presence of multiresistant strains is due to the inappropriate use of antibiotics on the property.