Browsing by Author "MARTINS, Daniele dos Santos"

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Características de células fibroblast-like derivadas do cultivo do saco vitelino em diferentes períodos gestacionais
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) WENCESLAU, Cristiane Valverde; MARTINS, Daniele dos Santos; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luiz Resende; AGRESTE, Fernanda R.; MORINI, Adriana Caroprezo; MORINI JÚNIOR, João Carlos; CARVALHO, Ana Flávia de; MIGLINO, Maria Angélica
Based on the knowledge regarding the erythropoietic activity of the vitelline membrane, which is considered the first hematopoietic and vascular site that appears in mammals during organogenesis, added to the erythropoiesis support function performed by mesenchymal stem cells, the present work aims to verify the cell expansion of the yolk sac using the explant technique, in view of the cell niches found in the fetal annex. For the cultivation of the yolk sac, 5 bitches with gestational ages of 30, 40 and 60 days were used, and were checked by ultrasound and Crow - Rump of each fetus according to Evans & Sack (1979). The bitches were submitted to ovariosalpingohisterectomy to collect the fetal attachment. All material was collected at the Faculty of Veterinary Medicine and Animal Science at the University of São Paulo - USP. The yolk sac was fragmented manually (mechanical fragmentation), with the aid of a scalpel blade until obtaining a homogenate of tissue fragments and cell groups, which were implanted in the 5 ml culture bottle with the modified Eagle Dulbeco medium - DMEM High with 10% fetal serum, streptomycin and penicillin. The cells were incubated at 38.5 degrees Celsius, at 100% humidity and 5% CO 2. The morphological description of the cell culture was performed periodically using the Nikon TS100 phase contrast microscope. The cells maintained in these conditions grew in the form of monolayer, with different fusiform cell types and large cells with cytoplasmic extensions like fibroblast-like.
Caracterização da morfologia externa de fetos de Paca
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) FRANCIOLLI, André Luis Rezende; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele dos Santos; MANÇANARES, Celina Almeida Furlanetto; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde; COSTA, Gerlane M.; MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; MIGLINO, Maria Angélica
The present scientific investigation aimed to study the embryonic development of pacas (Agouti Paca), with a view to the external morphological description of fetuses. Five fetuses were used, three of which were donated from the donation maintained by the Faculty of Agricultural and Veterinary Sciences of Jaboticabal - UNESP and two donated from the collection of biological material from FMVZ - USP. Fetuses 1 and 2 with 5.7 and 6.8 cm of Crow Rump, respectively, presented as morphological characteristics: marked facial immaturity, with the eyes covered by a prominent lens; the earlobes had a low level of development. The mouth had a small opening, where the formation of the incisor teeth was observed. Fetuses 3 and 4 with 9.6 and 10.5 cm of Crow Rump, respectively, showed well-developed ears, thoracic and pelvic limbs were in an equal degree of development with the presence of claws and development of facial features, like vibrissae around the nasal edges, the eyes remain protected by a prominent lens and the mouth contained the incisor teeth in an intermediate stage of development. Fetus 5, with 15.5 cm of Crow Rump, presented as a striking feature, the body covered by hair, thoracic and pelvic limbs in an equal degree of development with claws, development of facial characteristics, such as vibrissae, prominent eyes and well-developed ears. In all fetuses, the genital tubercle and the anal rim can be seen in the perineal region.
O efeito da própolis e da membrana amniótica no tratamento de queimaduras provocadas em ratos: um estudo macro e microscópico
(Fundação de Ensino Octávio Bastos, 2008-11-30) PESSOLATO, Alícia Greyce Turatti; MARTINS, Daniele dos Santos; MANÇANARES, Celina Almeida Furlanetto; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flavia de
The present work aimed to study macro and microscopically the dermal and epidermal response in the regenerative or healing repair process of rats treated daily with different and alternative local dressings. After anesthesia, a burn with a plate heated to 130 ° C for five seconds in 29 rats that were divided into three groups, being a control group treated with sterile saline (n = 9), group treated with propolis ointment (n = 10) and group treated with amniotic membrane (n = 10). The animals were euthanized at seven, 14 and 21 days of treatment. Macro and microscopically on the seventh day after injury, animals treated with propolis already showed significant acceleration of the healing process. The granulation tissue was evident and chronologically more advanced than in the control group, where the granulation tissue was more evident on the 21st day after burning, while the amnion-treated group, despite not accelerating the healing process, did not show inflammatory cells. since the first days of application. Both treatments studied were effective in treating burns compared to the control group. The 5% propolis ointment accelerated the local tissue repair process and therefore anticipated healing in the initial period macroscopically. The amniotic membrane stimulated tissue cell regeneration at the injured site and inhibited the inflammatory reaction on all days following the injuries.
Estudo da morfologia e vascularização do rim de avestruz
(Fundação de Ensino Octávio Bastos, 2001-11-30) MARTINS, Daniele dos Santos; AMBRÓSIO, Carlos Eduardo; MIGLINO, Maria Angélica; FINAZZI JÚNIOR, Renato; FERREIRA, Guilherme J. B.C.; BARREIRO, Rodrigo H.
The main objective of this work is to describe the morphology and vascularization of the ostrich kidney, making it possible to compare it with the domestic rooster, duck and goose kidney with that bird. The morphological study shows that these kidneys have a cranial lobe, a middle lobe and a caudal lobe. sciatic artery. The venous system corresponds to the renal portal system, which presents two amastomoses, in the ventral view; one occurs at the interposition of the middle lobe with the caudal lobe, and another located in the cranial lobe; the veins that drain the lobes are: cranial renal vein, middle renal vein and caudal renal vein.
Estudo da pluripotencialidade do fígado fetal canino nos diferentes períodos gestacionais
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) VIEIRA, Eduardo; MARTINS, Daniele dos Santos; WENCESLAU Cristiane Valverde; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de; ROSA, Ricardo Alexandre; MIGLINO, Maria Angélica
The study of the differentiation capacity of some cells, called stem cells or '' sistem '' cells represents an important tool for understanding and developing new research, as well as an enormous potential for discovering the treatment of numerous diseases. These embryonic stem cells are pluripotent, capable of multiplication and differentiation, and can proliferate in several cell types depending on the culture conditions. The identification of factors that lead to the direction of the cell differentiation process will allow, from embryonic stem cells, the culture to be carried out in a controlled manner among the most different cell types, making bioengineering possible. In this work, cells of the canine fetal liver were studied. The liver performs an important hematopoietic function during fetal development, while in the adult it has a multiplicity of functions such as: bile excretion, storage, synthesis and biotransformation. The livers, for study, were obtained from fetuses with gestational age with an average of 25, 35, 45 and 55 days, from females of mixed breed dogs that underwent ovariosalpingohesterectomy. These canine fetal liver cells were analyzed histologically to characterize cell differentiation, using previously established histological methods.