Browsing by Author "MARTINS, Daniele"

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    Análise morfológica das células do aspirado medular de fetos caninos
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) MÁRIO, Lara Carolina; BORGHESI, Jéssica; MELLO, Ana Carolina Landentin; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde
    The adult bone marrow is a source of stem cells (CT), which include the hematopoietic stem cells (HSC) that originate the entire definitive hematopoietic lineage; and mesenchymal stem cells (MSC), and or stromal which are responsible for providing a favorable environment for hematopoiesis. In addition to these main cell types, bone marrow is composed of several parents, differentiated cells and an extracellular matrix. This environment where CT is found is called a niche, as it houses a large amount of CT and cellular progenitors that are kept quiescent, acting normally and or in the face of injuries. However, little is known about the fetal bone marrow niche. It is known that the bone marrow niche is derived during the ontogenesis of the yolk sac, which subsequently migrates to various hematopoietic organs such as the placenta and liver, ultimately settling in the bone marrow. Thus, the present work aims to describe morphologically the cell types of canine fetal bone marrow at 40 and 60 days of gestation. For this purpose, 6 pregnant female uteri were used to collect the medullary aspirate from the femur bone and later structural and ultrastructural morphological analysis. The results demonstrated that the canine fetal bone marrow at 60 days of gestation has cell types similar to that of the adult bone marrow as well as CT, thus being able to be used as a source of CT isolation.
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    Estabelecimento das condições de isolamento e cultivo das células tronco mesenquimais da médula óssea em fetos de cães
    (Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) BORGHESI, Jéssica; MARIO, Lara Carolina; MELLO, Ana Carolina Landentin; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde
    Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into multiple differentiated terminal strains, including bone, cartilage, muscle, nerve, adipose and marrow stromal cells in vivo and in vitro. These cells are in addition to being isolated from the adult bone marrow and can be isolated from the fetal bone marrow. However, there is little study regarding the isolation technique of MSCs from fetal bone marrow. In this way, the work proposed to isolate MSC from canine fetuses based on the established methodology for obtaining MSC from adult human bone marrow. For this purpose, 12 pregnant female uteri were used to collect the medullary aspirate from the femur bone. The culture medium of choice was alpha-MEM and 15% fetal bovine serum, which provided the isolation of positive CTM-CD44.