Browsing by Author "FRANCIOLLI, André Luis Rezende"

Now showing 1 - 4 of 4

Análise microscópica e ultraestrutura do saco vitelino de equinos com até 47 dias de gestação
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2010-05-30) FERRAZ, Ana Claudia Cristiane; NASCIMENTO, Priscila Leal do; MANÇANARES, Celina de Almeida Furlanetto; FRANCIOLLI, André Luis Rezende; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de
This work aimed to describe microscopically the yolk sac of horses up to 47 days of gestation. To carry out this research, 37 placentas and embryos from 15 to 47 days of gestation were collected. The material was taken from adult, mixed breed mares in a slaughterhouse. Embryonic age was estimated according to the methodology used by EVANS SACK (1973). The embryos were destined for microscopic analysis. For analysis under light microscopy the fragments of the yolk sac membranes were fixed in 10% formaldehyde and Bouin followed by routine paraffin processing (TOLOSA et al; 2003). Serial slices with a thickness of 5μm were obtained in a microtome (Leica 2165®) which were submitted to staining by the methods of hematoxylin and eosin, picrosirius, Toluidine blue and Masson's trichrome (TOLOSA et al; 2003). Another part of the material was submitted to transmission and scanning electron microscopy. The yolk sac epithelium varied from globular to columnar, with uni or binucleated cells in a single layer, with a large amount of rough endoplasmic reticulum, distributed evenly, the epithelium supported on the mesenchyme forming vascular islands filled with hemangioblasts, being non-existent in animals older than 47 days of gestation; and characteristics of protein secretion.
Caracterização da morfologia externa de fetos de Paca
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) FRANCIOLLI, André Luis Rezende; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele dos Santos; MANÇANARES, Celina Almeida Furlanetto; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde; COSTA, Gerlane M.; MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; MIGLINO, Maria Angélica
The present scientific investigation aimed to study the embryonic development of pacas (Agouti Paca), with a view to the external morphological description of fetuses. Five fetuses were used, three of which were donated from the donation maintained by the Faculty of Agricultural and Veterinary Sciences of Jaboticabal - UNESP and two donated from the collection of biological material from FMVZ - USP. Fetuses 1 and 2 with 5.7 and 6.8 cm of Crow Rump, respectively, presented as morphological characteristics: marked facial immaturity, with the eyes covered by a prominent lens; the earlobes had a low level of development. The mouth had a small opening, where the formation of the incisor teeth was observed. Fetuses 3 and 4 with 9.6 and 10.5 cm of Crow Rump, respectively, showed well-developed ears, thoracic and pelvic limbs were in an equal degree of development with the presence of claws and development of facial features, like vibrissae around the nasal edges, the eyes remain protected by a prominent lens and the mouth contained the incisor teeth in an intermediate stage of development. Fetus 5, with 15.5 cm of Crow Rump, presented as a striking feature, the body covered by hair, thoracic and pelvic limbs in an equal degree of development with claws, development of facial characteristics, such as vibrissae, prominent eyes and well-developed ears. In all fetuses, the genital tubercle and the anal rim can be seen in the perineal region.
Influência do estresse causado pela tosquia e aumento de peso ao nascer de cordeiros
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) MORINI JÚNIOR, João Carlos; MORINI, Adriana Caroprezo; FRANCIOLLI, André Luis Rezende; FAVARON, Phelipe Oliveira; WENCESLAU, Cristiane Valverde; RIBEIRO, Luiz Alberto Oliveira; MANÇANARES, Celina de Almeida Furlanetto; MIGLINO, Maria Angélica; BOMBONATO, Pedro Primo
Reproductive losses in the southern region of Brazil have been a major problem for sheep farmers and studies in the area report that the weight of lambs at birth when greater than 4Kg allows it to survive during the winter period in which they are born. Due to the need for greater weight at birth, to ensure greater survival, numerous procedures have been performed in an attempt to promote lower mortality. The purpose of this work was to verify the influence of shearing on transplacental glycogen transport and its supposed relationship with the increase in birth weight in lambs (Ovies aries). For the realization of the project 9 sheep of the Australian Merino breed were used which were raised at the Federal University of Rio Grande do Sul - Porto Alegre / RS) - UFRGS in collaboration with the Lutheran Brazilian University - ULBRA. The animals were divided into 2 groups: the first with 5 sheared animals (OVE) at 70 days of the perinatal period and the second with four animals that were kept as control (OVC). The fetuses were collected through a surgical cesarean section performed at the end of pregnancy. For comparative purposes, placental and fetal weight were initially measured; to characterize the placenta, the number of placentons and the length, width and thickness of the placentonium were quantified, with the aid of a caliper. The collected placental material was fixed, dehydrated, diaphanized and included in paraffin, for later making slides and quantifying glycogen. As a partial macroscopic result, the placentas of the sheep in the sheared group had a weight 13% greater than those in the control group, in the fetuses the difference was more expressive, the weight of the fetuses sampled in the sheared group was 25% greater than those in the second group. The differences between the measures of thickness, length and width were insignificant, but always with higher rates in the tested group.
Membranas embrionárias e fetais de equinos sob análise de microscopia eletrônica de transmissão
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2009-09-30) NASCIMENTO, Priscila Leal do; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; FRANCIOLLI, André Luis Rezende; FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Ana Carolina Furlanetto; CARVALHO, Ana Flávia de
To develop this research, 16 pregnant uteri were used, of which 16 placentas were obtained divided into groups of different stages of development. The material was collected from adult, mixed breed mares up to 100 days of gestation. The membranes were intended for ultra-structural analysis and the results obtained under transmission electron microscopy (MET) were similar to those found by ASSIS-NETO (2005), in bovine membranes, and ITURRIZAGA (2005), in llamas. The fetal membranes did not show relevant structural differences in the different periods studied. The chorion showed columnar epithelium; mesenchymal tissue rich in collagen fibers, the trophoblastic cells being uni, binucleated and rarely trinucleated. A large number of mitochondria were present mainly at the apex of cells. The allantoic presented a simple columnar epithelium with globular nuclei and a large concentration of mitochondria at the cell apex, in the cell base there was a large amount of rough endoplasmic reticulum. The amnion showed a simple, squamous epithelium and at the cellular apex there was a large amount of electrondense granules covered by a rounded halo. The yolk sac epithelium varied from globose to columnar, with uni or binucleate cells in a single layer, with a large amount of rough endoplasmic reticulum, uniformly distributed. Between the endodermal cells, intercellular spaces were found. So far, it is concluded that the mare's placenta is choroidal alloid, epithelial, diffuse, non-deciduous and villous, with corium cells and a yolk sac with characteristics of protein secretion, while amnion presented secretion vesicles, but with less amount of rough endoplasmic reticulum.