Browsing by Author "CARVALHO, Ana Flávia de"

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Achado histopatológico de lipidose hepática em fetos de gambá (Didelphis sp.) lactantes
(Fundação de Ensino Octávio Bastos, 2008-11-30) GARCIA, Fernanda Figueiredo; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; ROSA, Ricardo Alexandre; CARVALHO, Ana Flávia de
Opossums (Didelphis sp.) Are highly reproducible marsupials, have a gestation period of approximately 12 days and extensive lactation. Fetuses receive high levels of carbohydrates and lipids from the mother during lactation. The objective of this work was describe the macro and microscopic morphology of the livers of opossum fetuses (Didelphis sp.) at different stages of their postnatal development. An old animal (5 days, 15 days, 22 days and 35 days) for macroscopic and microscopic verification of liver structures and consequent comparison with other domestic species. The animals' livers were processed and included by paraffin embedding techniques. Each block was cut and the sections were stained with HE, picrosirius, PAS histochemical reaction with hematoxylin, toluidine blue and Masson's trichrome to observe the hepatic structures. The macro and microscopic results indicate a premature development of the species in the fetal stage, similar to domestic animals and interestingly there is presence of hepatic lipidosis in all animals.
Alterações no perfil esparmático e constituição histológica dos testículos de coelhos sob a influência do aumento transitório da temperatura testicular
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2004-09-30) MARTINS, João Flávio Panattoni; BARROS, Michele Andrade de; IVO, Marcos Alexandre; OLIVEIRA, Priscila Carvalho de; BALIEIRO, Cristiano de Carvalho; AMBRÓSIO, Carlos Eduardo; MIGLINO, Maria Angélica; CARVALHO, Ana Flávia de
This study was conducted to investigate the sperm profile of rabbits, evaluating concentration, motility vigor and incidence of sperm with changes in the compaction of genetic material (pathologies of the DNA protein complex), under normal conditions and under the influence of the transient increase in temperature testicular. Eight fertile rabbits were used evaluating the parameters of 3 ejaculates on alternate days and then the animals were subjected to an increase in testicular temperature for 3 days, collecting semen samples on Mondays, Wednesdays and Fridays for 38 days for analysis. . Every 5 days a rabbit underwent orchiectomy for histological evaluation of testicles and epididymis.
Anais do 10º Encontro Científico-Acadêmico UNIFEOB 2024 v.1
(2025-05-19) JUNQUEIRA, José Roberto Almeida; FURLANETTO, Patrícia Gomes; CARVALHO, Ana Flávia de; WAITZ, Inês; MARUDI, Rodrigo; ZILLO, Fabiola Rebessi
O objetivo deste compilado é divulgar anualmente os trabalhos apresentados no Encontro Científico -Acadêmico, valorizando e incentivando a pesquisa acadêmica, os projetos integrados, projetos de extensão e trabalhos de conclusão de curso como parte essencial do desenvolvimento do estudante.
Anais do 10º Encontro Científico-Acadêmico UNIFEOB 2024 v.2
(2025-05-19) JUNQUEIRA, José Roberto Almeida.; FURLANETTO, Patrícia Gomes; CARVALHO, Ana Flávia de; WAITZ, Inês; MARUDI, Rodrigo; ZILLO, Fabiola Rebessi
O objetivo deste elaborado é divulgar regularmente os trabalhos apresentados no Encontro Científico -Acadêmico, valorizando e incentivando a pesquisa acadêmica, os projetos integrados, projetos de extensão e trabalhos de conclusão de curso como parte essencial do desenvolvimento do estudante.
Anais do 10º Encontro Científico-Acadêmico UNIFEOB 2024 v.3
(2025-05-19) JUNQUEIRA, José Roberto Almeida.; FURLANETTO, Patrícia Gomes; CARVALHO, Ana Flávia de; WAITZ, Inês; MARUDI, Rodrigo; ZILLO, Fabiola Rebessi
O objetivo deste elaborado é divulgar regularmente os trabalhos apresentados no Encontro Científico -Acadêmico, valorizando e incentivando a pesquisa acadêmica, os projetos integrados, projetos de extensão e trabalhos de conclusão de curso como parte essencial do desenvolvimento do estudante.
Anais do 8º Encontro Científico-Acadêmico UNIFEOB 2022 v.2
(Centro Universitário Fundação de Ensino Octávio Bastos, 2023-04-11) JUNQUEIRA, José Roberto Almeida.; FURLANETTO, Patrícia Gomes; CARVALHO, Ana Flávia de
This publication brings together the abstracts presented at the 8th UNIFEOB Academic Scientific Meeting, which was held on November 18 and 19, 2022, in order to in person. These were intense days full of presentations of the Final Papers of Course (TCC), Integrated Projects, Extension Projects and Research Projects, including projects of the Institutional Scientific Initiation Program, completed or in progress, of our students. The annual scientific-academic production meeting is in line with the three UNIFEOB's training axes, namely: academic, professional and for life, and, in especially values academic training, as students have the opportunity to learn to dialogue in a diverse research environment, enriching exchanges and experiences of each participant. The axis of academic formation stands out, without neglecting the formations professional and for life; we guarantee the integral formation of the UNIFEOB student to the provide opportunities for the experience of scientific activities. With contemporary themes that link theory and practice, reflecting the project institutional pedagogical development of technical and attitudinal skills, the 8th edition of the event consolidates in our academic community the importance of research, interdisciplinary and integrated activities and projects and is recognized as a space for exchange of academic ideas and experiences!
Anais do 9º Encontro Científico-Acadêmico UNIFEOB 2023 v.1
(Centro Universitário Fundação de Ensino Octávio Bastos, 2024-05-30) JUNQUEIRA, José Roberto Almeida; FURLANETTO, Patrícia Gomes; CARVALHO, Ana Flávia de; ZILLO, Fabiola Rebessi
The purpose of this compilation is disclose, annually, the works presented at the Scientific-Academic Meeting, valuing and encouraging academic research, projects integrated, the projects of extension and the work of course completion as parts essential for the student development.
Anais do 9º Encontro Científico-Acadêmico UNIFEOB 2023 v.2
(Centro Universitário Fundação de Ensino Octávio Bastos, 2024-05-30) JUNQUEIRA, José Roberto Almeida; FURLANETTO, Patrícia Gomes; CARVALHO, Ana Flávia de; ZILLO, Fabiola Rebessi
The purpose of this compilation is disclose, annually, the works presented at the Scientific-Academic Meeting, valuing and encouraging academic research, projects integrated, the projects of extension and the work of course completion as parts essential for the student development.
Análise biométrica de embriões, fetos e das membranas placentárias de equinos.
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2008-11-30) FERRAZ, Ana Cláudia Cristiane; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de
For this study, embryos, fetuses and attached membranes of 23 concepts of mares from Frigorífico Miramar - Pelotas RS were used. Embryos and fetuses were analyzed for size and external developmental characteristics to analyze gestational age. The attached membranes were weighed and measured for later correlation with gestational ages. The results obtained so far indicate that the embryonic and fetal development of horses is very fast in the early stages of development, this being a critical period of placental implantation and the beginning of the endocrine activities of the placenta. The biometric data related to the umbilical cord are not progressive according to development, having shown great variations among the animals studied.
Análise da estrutura macro e microscópica das glândulas salivares parótidas em duas espécies de procionídeos:
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) SANTOS, Amilton César dos; BERTASSOLI, Bruno M.; OLIVEIRA, Vanessa Cristina de; SOUZA, Aline F.; OLIVEIRA, Franciliusa D.; ROSA, Ricardo Alexandre; SILVA, Jodonai B.; CARVALHO, Ana Flávia de; MANÇANARES, Celina Almeida Furlanetto
The coati (Nasua nasua, Linnaeus, 1766) with daytime habits and the hand-pelada (Procyon cancrivorus, G. Cuvier, 1798) with twilight and nighttime habits, are terrestrial mammal species, belonging to the procionid family that are widely found in the Brazilian territory. For this work, three coatis and two hand-peeled adults from the Scientific Creation-CECRIMPAS of the University Center of the Fundação de Ensino Octávio Bastos (IBAMA 02027.003731 / 04-76) were used. These animals had already been euthanized and used in other research prior to ours. For this research, the parotid salivary glands of coatis and hand-peeled were identified, dissected, extracted, processed and prepared for observation under the optical microscope in the hematoxylin and eosin stains, picrossirius and photocumented. Macroscopically in each of the coatis and hand-peeled, a pair of parotid glands located below the parotid-auricular muscle, ventral to the auricular cartilage, can be seen, where it is in contact with the mandibular glands that are partially covered by the parotid glands. Microscopically, the parotid glands are multilobulated and are constituted by serous acini (parotid) in the coatis. The lobes of the coatis and hand-peeled parotid glands have striated intralobular ducts, lined by simple prismatic epithelium, interlobular ducts that vary their epithelium from simple cubic to bi-stratified cubic and intercalated ducts lined by simple cubic epithelium. They are composed of serous acini, similarly to that of most domestic animals, men and rodents, and unlike the results found in young dogs, lambs and some carnivores such as the ferret that has mixed acinos and some carnivores, which have serous and mucous acinos in their constitution.
Análise macro e microscópica do pâncreas e fígado de gambá
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2004-09-30) CASALS, Juliana; BELLATINE, Tatiana; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de; MARTINS, João Flávio Panattoni; ROQUETO, Marco Antônio; MANÇANARES, Celina de Almeida Furlanetto; MIGLINO, Maria Angélica; HOFFMAN, Roberto
16 animals from natural death were used in different situations. The techniques used, often traumatic, were injection of 10% formaldehyde followed by injection of latex (blue for the veins and red for the arteries) in the jugular vein and abdominal aorta. The results showed that the liver was dome-shaped in the cranial abdominal region, being macroscopically composed of 5 lobes and histologically similar to other domestic animals. The pancreas is dispersed and has an irregular surface. Its vascularization consisted of pancreatic duodenal, cranial and caudal arteries. Histologically, it presented characteristics similar to the pancreas of domestic species.
Análise macroscópica do aparelho reprodutor masculino do ouriço cacheiro
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2007-09-30) CASALS, Juliana Barbosa; BARREIRO, Juliana Regina; ROSA, Ricardo Alexandre; MANÇANARES, Celina Almeida Furlanetto; FAVARON, Phelipe Oliveira; CARVALHO, Ana Flávia de; MIGLINO, Maria Angélica; MARQUES, Leandro de Oliveira; ANDRADE, Filipe Ian Bindez de; NASCIMENTO, Priscila Leal do; FERRAZ, Ana Cláudia Cristiane; AMBRÓSIO, Carlos Eduardo
Rodents comprise most of the mammalian class. There are currently 2,021 species, organized into 443 genera and 29 families. Hedgehogs (Coendou villosus), are animals that in captivity, reproduce with some ease and become tame. In the urban and metropolitan region their presence is becoming common and this can be identified by the number of accidents involving dogs in the yard, registered in veterinary clinics. This work aimed to describe macroscopically the reproductive apparatus of Coendou villosus (hedgehog) to better clarify the anatomy of this important organ in the wild rodent model, since very little is known about this species. We compared the results obtained with other rodent species already studied. For the macroscopic description of the male reproductive system, 2 animals from donations were used, these animals ended up dying after attack by dogs.
Análise macroscópica e microscópica da glândula prepucial do Quati
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) BERTASSOLI, Bruno Machado; SANTOS, Amilton César dos; OLIVEIRA, Vanessa Cristina de; MANÇANARES, Celina Almeida Furlanetto; FRANCIOLLI, André L. R.; CARVALHO, Ana Flávia de
The purpose of this research is to describe the nasua nasua foreskin gland. Eight coatis from the CECRIMPAS Scientific Creatory (UNIFEOB) were used, the preputial glands were analyzed, dissected and described macro and microscopically, in order to establish their morphofunctional classification and thus be able to compare them with those of other species already described in the literature. Glandular tissue samples were collected, stained (hematoxylin and eosin, Toluidine blue, and picrosirius) and analyzed using light microscopy. All results were compiled and photo-documented. In the mentioned species, this gland has as its main function the territorial demarcation. In other animals such as primates, deer and rodents these demarcating glands are located in different anatomical regions, such as between the eyes, in the metatarsal, perianal and sternal. In our result of biometric measurements, we obtained an average of 3.8 ± 1.41 cm in width, 3.15 ± 0.93 cm in height and 10.26 ± 1.89 cm in circumference, and histologically classified as the tubuloalveolar type, with holocrine secretion.
Análise microscópica do estágio de ossificação do gambá (DIDELPHIS SP.) em diferentes estágios fetais através da diafanização e do exame radiográfico
(Fundação de Ensino Octávio Bastos, 2008-11-30) GARCIA, Fernanda Figueiredo; MANÇANARES, Celina Almeida Furlanetto; ALVES, Jeferson Douglas Soares; ROSA, Ricardo Alexandre; MIGLINO, Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de
Opossums (Didelphis sp.) Are marsupials that have a very short gestation period, therefore, their offspring are born immature and will end their development in the mother's marsupium. Because they are very early in their development, in the fetal stage of these animals, they already have premature growth of their organs and tissues as a whole. The objective of this work was to describe the ossification stages of opossum fetuses (Didelphis sp.) At different postnatal stages, through diaphanization and radiographic examination. One animal of each age (5 days, 15 days, 22 days and 35 days) was used to verify ossification and consequent comparison with other domestic species. The results indicate a premature ossification of the species in the fetal stage, unlike domestic animals.
Análise microscópica e ultraestrutura do saco vitelino de equinos com até 47 dias de gestação
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2010-05-30) FERRAZ, Ana Claudia Cristiane; NASCIMENTO, Priscila Leal do; MANÇANARES, Celina de Almeida Furlanetto; FRANCIOLLI, André Luis Rezende; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de
This work aimed to describe microscopically the yolk sac of horses up to 47 days of gestation. To carry out this research, 37 placentas and embryos from 15 to 47 days of gestation were collected. The material was taken from adult, mixed breed mares in a slaughterhouse. Embryonic age was estimated according to the methodology used by EVANS SACK (1973). The embryos were destined for microscopic analysis. For analysis under light microscopy the fragments of the yolk sac membranes were fixed in 10% formaldehyde and Bouin followed by routine paraffin processing (TOLOSA et al; 2003). Serial slices with a thickness of 5μm were obtained in a microtome (Leica 2165®) which were submitted to staining by the methods of hematoxylin and eosin, picrosirius, Toluidine blue and Masson's trichrome (TOLOSA et al; 2003). Another part of the material was submitted to transmission and scanning electron microscopy. The yolk sac epithelium varied from globular to columnar, with uni or binucleated cells in a single layer, with a large amount of rough endoplasmic reticulum, distributed evenly, the epithelium supported on the mesenchyme forming vascular islands filled with hemangioblasts, being non-existent in animals older than 47 days of gestation; and characteristics of protein secretion.
Análise morfológica das células do aspirado medular de fetos caninos
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) MÁRIO, Lara Carolina; BORGHESI, Jéssica; MELLO, Ana Carolina Landentin; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; MARTINS, Daniele; CARVALHO, Ana Flávia de; WENCESLAU, Cristiane Valverde
The adult bone marrow is a source of stem cells (CT), which include the hematopoietic stem cells (HSC) that originate the entire definitive hematopoietic lineage; and mesenchymal stem cells (MSC), and or stromal which are responsible for providing a favorable environment for hematopoiesis. In addition to these main cell types, bone marrow is composed of several parents, differentiated cells and an extracellular matrix. This environment where CT is found is called a niche, as it houses a large amount of CT and cellular progenitors that are kept quiescent, acting normally and or in the face of injuries. However, little is known about the fetal bone marrow niche. It is known that the bone marrow niche is derived during the ontogenesis of the yolk sac, which subsequently migrates to various hematopoietic organs such as the placenta and liver, ultimately settling in the bone marrow. Thus, the present work aims to describe morphologically the cell types of canine fetal bone marrow at 40 and 60 days of gestation. For this purpose, 6 pregnant female uteri were used to collect the medullary aspirate from the femur bone and later structural and ultrastructural morphological analysis. The results demonstrated that the canine fetal bone marrow at 60 days of gestation has cell types similar to that of the adult bone marrow as well as CT, thus being able to be used as a source of CT isolation.
Análise ultra estrutural das membranas embrionárias e fetais de equinos
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2008-11-30) NASCIMENTO, Priscila Leal do; MANÇANARES, Celina Almeida Furlanetto; MIGLINO, Maria Angélica; AMBRÓSIO, Carlos Eduardo; CARVALHO, Ana Flávia de
To develop this research, 13 pregnant uteri were used, of which 13 placentas were obtained divided into groups at different stages of development. The material was taken from adult, mixed breed mares and at different gestation periods of up to 120 days and was destined for ultrastructural analysis. The results obtained from SEM (scanning electron microscopy) were similar to those found by ASSIS-NETO (2005), in bovine membranes. The allantoic showed cells whose surface was bordered by microvilli, forming polyhedral images, protruding “button” shaped structures were also observed. At the amnion, a surface similar to that of the allantois was observed, with its entire length covered by microvilli. In the corium, the apical surface of the cells are rounded and covered by microvilli. The yolk sac showed droplets of secretion at the apex of the cells indicating exocytosis. So far it has been concluded that the mare's placenta is chorioallantoic, epithelial, diffuse, non-deciduous and villous.
Anatomia microscópica do sistema reprodutor feminino do Tamanduá Bandeira
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) GARCIA, Rafael de Carvalho; BRANCO, Èrika Renata; LIMA, Ana Rita de; OLIO, Rennan Lopes; WENCESLAU, Cristiane Valverde; MANÇANARES, Celina Almeida Furlanetto; THOMAZ, Juliana Mantovani; CARVALHO, Ana Flávia de
The giant anteater is a large animal and can be very aggressive with its claws if disturbed, it belongs to the Xenarthra Order, Family Myrmecophagidae, which are toothless members, and therefore called edentats. Some reports have been made about this species, but many relevant data have not been published, emphasizing basic concepts of morphology and biometrics, which could be extremely useful for the development of reproductive biotechnologies, aiming at the preservation of the species. For this study, two animals donated by UFRA – PA were used, in which the microscopic characteristics of the female reproductive system were analyzed, in order to clarify the peculiar aspects of the species. The results obtained reveal that the ovaries, fallopian tubes and uterus are similar histologically to those of domestic animals.
Anormalidades na placenta bovina de clones e interferência no desenvolvimento embrionário fetal
(Centro Universitário da Fundação de Ensino Octávio Bastos, 2011-05-30) LUCIANO, Adriano Pedreira; SILVA, Marcos Donizete da; MIGLINO, Maria Angélica; CARVALHO, Ana Flávia de
Bovines are very important animals for the population of today, as they are an excellent source of protein, with this, several researches are carried out in order to improve the quality and to achieve greater productivity of a herd. New techniques for production and reproduction are important for the advancement of the bovine cut which is growing more and more. An example of this is the cloning of animals of high genetic value, through the transfer of somatic nucleus (NT). This technique causes high loss of fetuses and mortality at birth due to a syndrome, related to the development of the placenta.
Apoptose na placenta do búfalo
(Fundação de Ensino Octávio Bastos, 2001-11-30) PEREIRA, Flávia Thomaz Verechia; MIGLINO, Maria Angélica; PETRONI, Natália Luiza; CARVALHO, Ana Flávia de; DAGLI, Maria Lúcia Zaidan
The ruminant placenta has a very uniform macroscopic structure, based on specialized areas of apposition and proliferation of maternal fetal membranes: the placentones (LEISER et al, 1994; LEISER et al, 1997a; LEISER et al, 1997b). Showing a fundamental role for the success of pregnancy, these structures have been extensively studied in several species of domestic animals of commercial interest. In these areas of contact between mother and fetus, apoptosis (programmed cell death) occurs, appearing with 7-10 months of pregnancy, however the pregnancy of the buffalo is 10 months and apoptosis is visualized in these late stages of pregnancy.